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Animal Cell Biotechnology pp 489–507Cite as

Insect Cell Cultivation and Generation of Recombinant Baculovirus Particles for Recombinant Protein Production

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Part of the book series: Methods in Biotechnology ((MIBT,volume 24))

Abstract

Viral transduction of eukaryotic cell lines is one possibility to efficiently generate recombinant proteins, and among all viral-based expression systems the baculovirus/insect cell expression system (BEVS) is certainly the most well known and applied. This chapter delineates the individual process steps of the system: maintenance of insect cell cultures, transfection of recombinant DNA into different insect cell lines, amplification of virus stocks, virus titer determination, and accessory experiments required to give rise to optimal production conditions.

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Authors and Affiliations

  1. Novartis Institutes for BioMedical Research, Basel, Switzerland

    Sabine Geisse

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  1. Sabine Geisse
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Editors and Affiliations

  1. Institute for Bioprocess and Biochemical Engineering, Hamburg University of Technology, Hamburg, Germany

    Ralf Pörtner

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© 2007 Humana Press Inc., Totowa, NJ

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Geisse, S. (2007). Insect Cell Cultivation and Generation of Recombinant Baculovirus Particles for Recombinant Protein Production. In: Pörtner, R. (eds) Animal Cell Biotechnology. Methods in Biotechnology, vol 24. Humana Press. https://doi.org/10.1007/978-1-59745-399-8_23

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  • DOI: https://doi.org/10.1007/978-1-59745-399-8_23

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-660-3

  • Online ISBN: 978-1-59745-399-8

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