Summary
Substantial progress has been made over the last several years in the development of protocols for the isolation of large numbers of dendritic cells (DCs) from different tissues and their short-term culture. Indeed, several stable DC lines and clones have been established from various tissues of mice and humans, providing useful experimental tools for studying the biology of DCs at both molecular and biochemical levels and for the establishment of new DC-based immunotherapies. In this chapter, we will describe the development of long-term DC lines that maintain the growth factor dependence and their immature functional state, thus providing a unique opportunity to study the mechanisms of the initiation of the immune response to infectious agents.
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Acknowledgments
The authors wish to thank Renato Ostuni for his contribution in the setting up of the protocol of DC differentiation from BM, and Federica Mainoldi and Anna Ranghetti (Sekmed, s.r.l) for technical support.
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Mortellaro, A., Urbano, M., Citterio, S., Foti, M., Granucci, F., Ricciardi-Castagnoli, P. (2009). Generation of Murine Growth Factor-Dependent Long-Term Dendritic Cell Lines to Investigate Host-Parasite Interactions. In: Reiner, N. (eds) Macrophages and Dendritic Cells. Methods in Molecular Biology™, vol 531. Humana Press. https://doi.org/10.1007/978-1-59745-396-7_2
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DOI: https://doi.org/10.1007/978-1-59745-396-7_2
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