Abstract
HCV infection provides a classic example of the phenomenon of quasispecies. Because several lines of investigation support the contribution of quasispecies to HCV’s capacity to maintain a persistent infection, adequate characterization of the quasispecies is important. The hypervariable region 1 (HVR1) of the E2 glycoprotein has been particularly well studied in this regard. We present here a rapid method for characterizing the HVR1 quasispecies, based on in vitro coupled transcription/translation of the amplicons, followed by mass spectrometry of the resulting peptide mix.
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Acknowledgments
This work was supported by the Canadian Institutes of Health Research, The Canadian Liver Foundation, and the Department of Paediatric Laboratory Medicine, Hospital for Sick Children.
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC
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Yea, C., Ayers, M., Tellier, R. (2009). Characterization of HCV Quasispecies in the Hypervariable Region 1 (HVR 1) by In Vitro Translation and Mass Spectrometry. In: Tang, H. (eds) Hepatitis C. Methods in Molecular Biology™, vol 510. Humana Press. https://doi.org/10.1007/978-1-59745-394-3_6
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DOI: https://doi.org/10.1007/978-1-59745-394-3_6
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