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The Use of Two-Dimensional SDS-PAGE to Analyze the Glycan Heterogeneity of the Respiratory Syncytial Virus Fusion Protein

  • Terence P. McDonald
  • Richard J. Sugrue
Part of the Methods in Molecular Biology book series (MIMB, volume 379)

Abstract

The respiratory syncytial virus (RSV) fusion (F) protein is synthesized as an inactive precursor (F0), which subsequently undergoes post-translational cleavage to give the disulphide bond-linked F1 and F2 subunits. The methodology detailing the use of two-dimensional electrophoresis, endoglycosidases, and α-mannosidase inhibitors, as applied to investigating F protein glycan maturation, is given. Examples are used to show how this methodology was used to provide evidence for glycan heterogeneity within the mature F protein.

Key Words

Proteomics F protein respiratory syncytial virus deoxymannojirimycin Swainsonine 

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Copyright information

© Humana Press Inc., Totowa, NJ 2007

Authors and Affiliations

  • Terence P. McDonald
    • 1
  • Richard J. Sugrue
    • 1
  1. 1.MRC Virology UnitInstitute of VirologyGlasgowUK

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