Expression and Purification of Viral Glycoproteins Using Recombinant Vaccinia Viruses for Functional and Structural Studies

  • Zhu-Nan Li
  • David A. Steinhauer
Part of the Methods in Molecular Biology book series (MIMB, volume 379)


Methods for generating recombinant vaccinia viruses for the expression of foreign viral glycoproteins in mammalian cell lines and the purification of expressed viral glycoproteins are described. These methods are based on many years of experience with the influenza hemagglutinin glycoprotein (HA). However, they are applicable for studies on other viral glycoproteins, and with slight modifications, could be useful for cellular proteins as well.

Key Words

Viral glycoproteins recombinant vaccinia viruses influenza hemagglutinin structure function 


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Copyright information

© Humana Press Inc., Totowa, NJ 2007

Authors and Affiliations

  • Zhu-Nan Li
    • 1
  • David A. Steinhauer
    • 1
  1. 1.Emory University School of MedicineAtlanta

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