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Functional Analysis of the N-Linked Glycans Within the Fusion Protein of Respiratory Syncytial Virus

  • Ping Li
  • Helen W. McL. Rixon
  • Gaie Brown
  • Richard J. Sugrue
Part of the Methods in Molecular Biology book series (MIMB, volume 379)

Abstract

The respiratory syncytial virus fusion (F) protein is initially expressed as a single polypeptide chain (F0). The F0 subsequently undergoes posttranslational cleavage-by-cell protease activity to produce the F1 and F2 subunits. Each of the two subunits within the mature F protein is modified by the addition of N-linked glycans. The individual N-linked glycans on the F protein were selectively removed by using site-directed mutagenesis to mutate the individual glycan-acceptor sites. In this way the role of these individual glycans in targeting of the F protein to the cell surface, and on the ability of the F protein to induce membrane fusion, was examined.

Key Words

Respiratory syncytial virus F protein site-directed mutagenesis glycosylation metabolic labeling fusion activity surface expression 

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Copyright information

© Humana Press Inc., Totowa, NJ 2007

Authors and Affiliations

  • Ping Li
    • 1
  • Helen W. McL. Rixon
    • 1
  • Gaie Brown
    • 1
  • Richard J. Sugrue
    • 1
  1. 1.MRC Virology UnitInstitute of VirologyGlasgowUK

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