The C Type Lectins DC-SIGN and L-SIGN

Receptors for Viral Glycoproteins
  • Pierre-Yves Lozach
  • Laura Burleigh
  • Isabelle Staropoli
  • Ali Amara
Part of the Methods in Molecular Biology book series (MIMB, volume 379)


DC-SIGN and L-SIGN are C-type lectins that recognize carbohydrate structures present on viral glycoproteins and function as attachment factors for several enveloped viruses. DC-SIGN and L-SIGN enhance viral entry and facilitate infection of cells that express the cognate entry receptor (cis-infection). They are also able to capture viruses and transfer viral infections to other target cells (trans-infection). In this chapter, we will give an overview of protocols used to produce soluble viral glycoproteins at high levels and to study the molecular basis of viruses/DC-SIGN and L-SIGN binding and internalization. We will also describe techniques to investigate the molecular mechanisms by which DC-SIGN or L-SIGN spread viral infections.

Key Words

DC-SIGN L-SIGN dendritic cells endothelial cells viruses envelope glycoproteins endocytosis viral entry infection viral transmission 


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Copyright information

© Humana Press Inc., Totowa, NJ 2007

Authors and Affiliations

  • Pierre-Yves Lozach
    • 1
    • 2
  • Laura Burleigh
    • 1
  • Isabelle Staropoli
    • 1
  • Ali Amara
    • 1
  1. 1.Laboratoire de Pathogénie Virale MoléculaireInstitut PasteurParisFrance
  2. 2.UMR de Virologie Moléculaire et StructuraleCNRS 2472-INRA 1157Gif-sur-Yvette CedexFrance

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