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Expression of the Severe Acute Respiratory Syndrome Coronavirus 3a Protein and the Assembly of Coronavirus-Like Particles in the Baculovirus Expression System

  • Sehaam Khan
  • Mah-Lee Ng
  • Yee-Joo Tan
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 379)

Abstract

The Bac-to-Bac Baculovirus expression system was used to generate a recombinant baculovirus capable of expressing the severe acute respiratory syndrome (SARS)-coronavirus (CoV) 3a protein. Using the same expression system, two structural proteins, membrane (M) and envelope (E), were co-expressed to form SARS-CoV virus-like particles (VLPs) within an insect cell. Expression of viral proteins was confirmed by Western blot analysis and the formation of VLPs was studied by transmission electron microscopy.

Key Words

Bac-to-Bac Baculovirus expression system severe acute respiratory syndrome coronavirus SARS-CoV 3a protein membrane protein (M) envelope protein (E) virus-like particles (VLPs) transmission electron microscopy Western Blot 

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Copyright information

© Humana Press Inc., Totowa, NJ 2007

Authors and Affiliations

  • Sehaam Khan
    • 1
  • Mah-Lee Ng
    • 2
  • Yee-Joo Tan
    • 1
  1. 1.Collaborative Antiviral Research Group, Institute of Molecular and Cell BiologyProteosSingapore
  2. 2.Department of Microbiology and Electron Microscopy Unit, Faculty of MedicineNational University of SingaporeSingapore

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