Analysis of Glycoproteins of Viruses in the Family Bunyaviridae

  • Xiaohong Shi
  • Richard M. Elliott
Part of the Methods in Molecular Biology book series (MIMB, volume 379)


The membrane glycoproteins (Gn and Gc) of viruses in the family Bunyaviridae form projections on the virion envelope and are involved in virus entry and eliciting protective immunity. The glycoproteins are modified by N-linked glycosylation and accumulate in the Golgi complex where virions mature and bud. In this chapter, we describe the methods that have been used in our laboratory for the study of the glycoproteins of Bunyamwera virus, the prototype of the family. The protocols cover the expression of viral glycoproteins, examination of intracellular localization by immnunofluorescent confocal microscopy, radiolabeling, immunoprecipitation, and SDS-PAGE analysis of the proteins, and the improved reverse genetic system to rescue recombinant viruses that contain mutations at N-linked glycosylation sites.

Key Words

N-linked glycosylation Bunyaviridae Bunyamwera virus bunyavirus glycoprotein protein expression virus rescue and reverse genetics 


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Copyright information

© Humana Press Inc., Totowa, NJ 2007

Authors and Affiliations

  • Xiaohong Shi
    • 1
  • Richard M. Elliott
    • 1
  1. 1.Centre for Biomolecular Sciences, School of BiologyUniversity of St AndrewsSt AndrewsUK

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