Abstract
Microprojectile bombardment or biolistic techniques have been widely used for cereal transformation. These methods rely on the acceleration of gold particles, coated with plasmid DNA, into plant cells as a method of directly introducing the DNA. The first report of the generation of fertile, transgenic barley plants used biolistic techniques. However, more recently Agrobacterium-mediated transformation has been adopted as the method of choice for most cereals including barley. Biolistic procedures are still important for some barley transformation applications and also provide transient test systems for the rapid checking of constructs. This chapter describes methods for the transformation of barley using biolistic procedures and also highlights the use of the technology in transient assays.
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Wan, Y. and Lemaux, P. G. (1994) Generation of large numbers of independent transformed fertile barley plants.Plant Physiol. 104, 37–48.
Harwood, W. A., Ross, S. M., Cilento, P. and Snape, J. W. (2000) The effect of DNA/gold particle preparation technique, and particle bombardment device, on the transformation of barley (Hordeum vulgare). Euphytica 111, 67–76.
Yao, Q. A., Simion, E., William, M., Krochko, J. and Kasha, K. J. (1997) Biolistic transformation of haploid isolated microspores of barley (Hordeum vulgare L.).Genome 40, 570–581.
Zhang, S., Cho, M.-J., Koprek, T., Yun, R., Bregitzer, P. and Lemaux, P. G. (1999) Genetic transformation of commercial cultivars of oat (Avena sativa L.) and barley (Hordeum vulgare L.) using in vitro shoot meristematic cultures derived from germinating seedlings. Plant Cell Rep. 18, 959–966.
Cho, M.-J., Jiang, W. and Lemaux, P. G. (1998) Transformation of recalcitrant barley cultivars through improvement of regenerability and decreased albinism.Plant Sci. 138, 229–244.
Travella, S., Ross, S. M., Harden, J., Everett, C., Snape, J. W. and Harwood, W. A. (2005) A comparison of transgenic barley lines produced by particle bombardment and Agrobacterium-mediated techniques. Plant Cell Rep. 23, 780–789.
Agrawal, P. K., Kohli, A., Twyman, R. M. and Christou, P. (2005) Transformation of plants with multiple cassettes generates simple transgene integration patterns and high expression levels.Mol. Breed. 16, 247–260.
Harwood, W. A., Ross, S. M., Bulley, S. M., Travella, S., Busch, B., Harden, J. and Snape, J. W. (2002) Use of the firefly luciferase gene in a barley (Hordeum vulgare) transformation system. Plant Cell Rep. 21, 320–326.
Becker, D., Brettschneider, R. and Lorz, H. (1994) Fertile transgenic wheat from microprojectile bombardment of scutellar tissue.Plant J. 5, 299–307.
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC
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Harwood*, W., Smedley, M. (2009). Barley Transformation Using Biolistic Techniques. In: Jones, H., Shewry, P. (eds) Transgenic Wheat, Barley and Oats. Methods in Molecular Biology™, vol 478. Humana Press. https://doi.org/10.1007/978-1-59745-379-0_8
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DOI: https://doi.org/10.1007/978-1-59745-379-0_8
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