Abstract
This chapter details a reproducible method for the transformation of spring wheat using Agrobacterium tumefaciens via the direct inoculation of bacteria into immature seeds in planta as described in patent WO 00/63398(1. Transformation efficiencies from 1 to 30% have been obtained and average efficiencies of at least 5% are routinely achieved. Regenerated plants are phenotypically normal with 30–50% of transformation events carrying introduced genes at single insertion sites, a higher rate than is typically reported for transgenic plants produced using biolistic transformation methods.
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Risacher, T., Craze, M., Bowden, S., Paul*, W., Barsby, T. (2009). Highly Efficient Agrobacterium-Mediated Transformation of Wheat Via In Planta Inoculation. In: Jones, H., Shewry, P. (eds) Transgenic Wheat, Barley and Oats. Methods in Molecular Biology™, vol 478. Humana Press. https://doi.org/10.1007/978-1-59745-379-0_7
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DOI: https://doi.org/10.1007/978-1-59745-379-0_7
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