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Selection of Transformed Plants

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Transgenic Wheat, Barley and Oats

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 478))

Abstract

The low frequency and randomness of transgene integration into host cells, combined with the significant challenges of recovering whole plants from those rare events, makes the use of selectable marker genes routine in plant transformation experiments. For research applications that are unlikely to be grown in the field, strong herbicide- or antibiotic resistance is commonly used. Here we use genes conferring resistance to glufosinate herbicides as an example of a selectable marker in wheat transformation by either Agrobacterium or biolistics.

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Acknowledgements

Rothamsted Research receives grant-aided support from the Biotechnology and Biological Sciences Research Council (BBSRC) of the UK.

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  1. Centre for Crop Genetic Improvement, Department of Plant Sciences, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, UK

    Huw D. Jones* & Caroline A. Sparks

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  1. Huw D. Jones*
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    © 2009 Humana Press, a part of Springer Science+Business Media, LLC

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    Jones*, H., Sparks, C. (2009). Selection of Transformed Plants. In: Jones, H., Shewry, P. (eds) Transgenic Wheat, Barley and Oats. Methods in Molecular Biology™, vol 478. Humana Press. https://doi.org/10.1007/978-1-59745-379-0_2

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    • DOI: https://doi.org/10.1007/978-1-59745-379-0_2

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