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Capillary Electrochromatography and On-Line Concentration

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Capillary Electrophoresis

Part of the book series: Methods In Molecular Biology™ ((MIMB,volume 384))

Summary

Capillary electrochromatography (CEC) is a micro-separation technique that combines the advantages of capillary zone electrophoresis with those of high-performance liquid chromatography. Accordingly, it has attracted extensive attention over the last decade. Among the stationary phases for CEC, monolithic stationary phase has been regarded as the most suitable stationary phase for CEC because of its simple preparation, the elimination of frits, and its excellent performance. In this chapter, procedures for preparing CEC monolithic columns with an improved configuration, in which there are stationary phases at both sides of detection window and no stationary phase at detection window, are presented. The separation of acidic and basic compounds on such monolithic columns is used as an example to demonstrate CEC separation protocol. Additionally, an on-line concentration technique in CEC is presented. As a result of the coexistence of stationary phase and electric field in a CEC column, it is possible to employ chromatographic zone sharpening and field-amplified sample stacking effects simultaneously to improve CEC detection sensitivity.

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Acknowledgments

The present work is partially supported by National Natural Science Foundation of China (No. 20105006) and 973 Project of the Ministry of Science and Technology of China (No. 001CB510202), a grant form the New Energy and Industrial Technology Development Organization of the Ministry of Economy, Trade and Industry, the CREST program of the Japan Science and Technology Corporation, a Grant-in-Aid for Scientific Research from the Ministry of Health and Welfare, a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Technology, and a Grant-in-aid of the 21st Century COE program from the Ministry of Education, Science and Technology, Japan.

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Authors and Affiliations

  1. Department of Molecular and Pharmaceutical Biotechnology, Graduate School of Pharmaceutical Sciences, The University of Tokushima, Tokushima, Japan

    Guichen Ping & Yoshinobu Baba

  2. HelmholtzZentrum München, German Research Center for Environmental Health, Neuherberg, Germany

    Philippe Schmitt-Kopplin

  3. Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China

    Yukui Zhang

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  1. Guichen Ping
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  1. HelmholtzZentrum München German Research Center for Environmental Health, Neuherberg, Germany

    Philippe Schmitt-Kopplin

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Ping, G., Schmitt-Kopplin, P., Zhang, Y., Baba, Y. (2008). Capillary Electrochromatography and On-Line Concentration. In: Schmitt-Kopplin, P. (eds) Capillary Electrophoresis. Methods In Molecular Biology™, vol 384. Humana Press. https://doi.org/10.1007/978-1-59745-376-9_31

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  • DOI: https://doi.org/10.1007/978-1-59745-376-9_31

  • Publisher Name: Humana Press

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