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Analysis of Proteins by Capillary Electrophoresis

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Capillary Electrophoresis

Part of the book series: Methods In Molecular Biology™ ((MIMB,volume 384))

Summary

This chapter describes the basic principles of protein analysis by capillary electrophoresis, and provides an overview of the literature and thus a comprehensive summary of special topics in this field. The “Materials and Methods” section includes the main experimental points to be taken into consideration, namely, sample pretreatment, reduction of protein adsorption to capillary wall, increase of selectivity, detection modes, and special electrophoretic modes. Because there are many capillary electrophoretic methods for protein analysis, not all experimental steps are listed; however, the main references are cited. Additional experimental information can be found in the Notes. Finally, an overview of the most relevant applications, divided according to the origin of the samples into human proteins, food and agricultural products, pharmaceutical proteins, proteome, and special proteins, is given with the relevant literature.

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References

  1. Righetti, P. G., Hamdan, M., Antonucci, F., Verzola, B., and Bossi, A. (2004) Electrophoresis of proteins and peptides. J. Chromatogr. Libr. 69B, 633–668.

    CAS  Google Scholar 

  2. Ferranti, P. (2004) Mass spectrometric approach for the analysis of food proteins. Eurp. J. Mass Spectrom. 10, 349–358.

    CAS  Google Scholar 

  3. Bossuyt, X. (2004) Interferences in clinical capillary zone electrophoresis of serum proteins. Electrophoresis 25, 1485–1487.

    CAS  PubMed  Google Scholar 

  4. Ramstrom, M. and Bergquist, J. (2004) Miniaturized proteomics and peptidomics using capillary liquid separation and high resolution mass spectrometry. FEBS Lett. 567, 92–96.

    CAS  PubMed  Google Scholar 

  5. Hernandez-Borges, J., Neusuess, C., Cifuentes, A., and Pelzing, M. (2004) On-line capillary electrophoresis-mass spectrometry for the analysis of biomolecules. Electrophoresis 25, 2257–2281.

    CAS  PubMed  Google Scholar 

  6. Righetti, P. G. (2004) Bioanalysis: its past, present, and some future. Electrophoresis 25, 2111–2127.

    CAS  PubMed  Google Scholar 

  7. Pierceall, W. E., Zhang, L., and Hughes, D. E. (2004) Affinity capillary electrophoresis analyses of protein-protein interactions in target-directed drug discovery. Meth. Mol. Biol. 261, 187–197.

    CAS  Google Scholar 

  8. Hutterer, K. and Dolnik, V. (2003) Capillary electrophoresis of proteins {2001–2003.} Electrophoresis 24, 3998–4012.

    CAS  PubMed  Google Scholar 

  9. Huck, C. W., Stecher, G., Bakry, R., and Bonn, G. K. (2003) Recent progress in high-performance capillary bioseparations. Electrophoresis 24, 3977–3997.

    CAS  PubMed  Google Scholar 

  10. Guttman, A. (2003) Gel and polymer-solution mediated separation of biopolymers by capillary electrophoresis. J. Chromatogr. Sci. 41, 449–459.

    CAS  PubMed  Google Scholar 

  11. Woods, L. A. and Ewing, A. G. (2003) Analysis of single mammalian cells. Anal. Bioanal. Chem. 376, 281–283.

    CAS  PubMed  Google Scholar 

  12. Lamari, F. N. and Karamanos, N. K. (2003) Methodological challenges of protein analysis in blood serum and cerebrospinal fluid. Chromatographia 58, 349–356.

    CAS  Google Scholar 

  13. Petersen, J. R., Okorodudu, A. O., Mohammad, A., and Payne, D. A. (2003) Capillary electrophoresis and its application in the clinical laboratory. Clin. Chim. Acta 330, 1–30.

    CAS  PubMed  Google Scholar 

  14. Rochu, D. (2003) Capillary electrophoresis for exploring protein stability. BIOforum Eur. 7, 37–39.

    CAS  Google Scholar 

  15. Lacher, N. A., Garrison, K. E., Martin, R. S., and Lunte, S. M. (2001) Microchip capillary electrophoresis/electrochemistry. Electrophoresis 22, 2526–2536.

    CAS  PubMed  Google Scholar 

  16. Dolnik, V. and Hutterer, K. M. (2001) Capillary electrophoresis of proteins {1999–2001.} Electrophoresis 22, 4163–4178.

    CAS  PubMed  Google Scholar 

  17. Righetti, P. G., Gelfi, C., Bossi, A., et al. (2000) Capillary electrophoresis of peptides and proteins in isoelectric buffers: an update. Electrophoresis 21, {4046–4053.}

    CAS  PubMed  Google Scholar 

  18. Krylov, S. N. and Dovichi, N. J. (2000) Capillary electrophoresis for the analysis of biopolymers. Anal. Chem. 72, 11R–128R.

    Google Scholar 

  19. Manabe, T. (1999) Capillary electrophoresis of proteins for proteomic studies. Electrophoresis 20, 3116–3121.

    CAS  PubMed  Google Scholar 

  20. Dolnik, V. (1999) Recent developments in capillary zone electrophoresis of proteins. Electrophoresis 20, 3106–3115.

    CAS  PubMed  Google Scholar 

  21. Stroink, T., Paarlberg, E., Waterval, J. C. M., Bult, A., and Underberg, W. J. M. (2001) On-line sample preconcentration in capillary electrophoresis, focused on the determination of proteins and peptides. Electrophoresis 22, 2375–2383.

    CAS  PubMed  Google Scholar 

  22. Guzman, N. A. and Stubbs, R. J. (2001) The use of selective adsorbents in capillary electrophoresis-mass spectrometry for analyte preconcentration and microreactions: a powerful three-dimensional tool for multiple chemical and biological applications. Electrophoresis 22, 3602–3628.

    CAS  PubMed  Google Scholar 

  23. Neususs, C., Pelzing, M., and Macht, M. (2002) A robust approach for the analysis of peptides in the low femtomole range by capillary electrophoresis-tandem mass spectrometry. Electrophoresis 23, 3149–3159.

    CAS  PubMed  Google Scholar 

  24. Tseng, W. L. and Chang, H. T. (2001) Regulation of electroosmotic flow and electrophoretic mobility of proteins for concentration without desalting. J. Chromatogr. A. 924, 93–101.

    CAS  PubMed  Google Scholar 

  25. German, I., Roper, M. G., Kalra, S. P., Rhineart, E., and Kennedy, R. T. (2001) Capillary liquid chromatography of multiple peptides with on-line capillary electrophoresis immunoassay detection. Electrophoresis 22, 3659–3667.

    CAS  PubMed  Google Scholar 

  26. Pospichal, J. and Glovinova, E. (2001) Analytical aspects of carrier ampholyte-free isoelectric focusing. J. Chromatogr. A 918, 195–203.

    CAS  PubMed  Google Scholar 

  27. Macounova, K., Carbera, C. R., and Yager, P. (2001) Concentration and separation of proteins in microfluidic channels on the basis of transverse IEF. Anal. Chem. 73, 1627–1633.

    CAS  PubMed  Google Scholar 

  28. Copper, J.W.; Gao, J.and Lee, C.S. (2002) Gel Protein Capillary Extraction Apparatus. Electronic Protein Transfer. Anal. Chem. 74, 1182–1186.

    Google Scholar 

  29. Larsson, M. and Lutz, E. S. M. (2000) Transient isotachophoresis for sensitivity enhancement in capillary electrophoresis-mass spectrometry for peptide analysis. Electrophoresis 21, 2859–2865.

    CAS  PubMed  Google Scholar 

  30. Huck, C. W. and Bonn, G. K. (2000) Review: polymer based sorbents for solid-phase extraction. J. Chromatogr. A 885, 51–72.

    CAS  PubMed  Google Scholar 

  31. Waterval, J. C., Hommels, G., Teeuwsen, J., Bult, A., Lingeman, H., and Underberg, W. J. (2000) Transient isotachophoresis for sensitivity enhancement in capillary electrophoresis-mass spectrometry for peptide analysis. Electrophoresis 21, 2851–2858.

    CAS  PubMed  Google Scholar 

  32. Zhang, Z., Carpenter, E., Puyan, X. L., and Dovichi, N. J. (2001) Manipulation of protein fingerprints during on-column fluorescent labeling: protein fingerprinting of six Staphylococcus species by capillary electrophoresis. Electrophoresis 22, 1127–1132.

    CAS  Google Scholar 

  33. Hansen, L. C. and Sievers, R. E. (1974) Highly permeable open-pore polyurethane columns for liquid chromatography. J. Chromatogr. 99, 123–133.

    CAS  Google Scholar 

  34. Hjerten, S., Liao, J. -L., and Zhang, R. (1974) High-performance liquid chromatography on continuous polymer beds. J. Chromatogr. 473, 273–275.

    Google Scholar 

  35. Benito, I., Marina, M. L., Saz, J. M., and Diez-Masa, J. C. (1999) Detection of bovine whey proteins by on-column derivatization capillary electrophoresis with laser-induced fluorescence monitoring. J. Chromatogr. A 841, 105–114.

    CAS  PubMed  Google Scholar 

  36. Horka, M. and Slais, K. (2002) Pyrenebutanoate as a dynamic protein modifier for fluorometric detection in capillary zone electrophoresis. Electrophoresis 23, 1090–1095.

    CAS  PubMed  Google Scholar 

  37. Tan, W., Fan, Z. H., Qiu, C. X., Ricco, A. J., and Gibbons, I. (2002) New technique for capillary electrophoresis directly coupled with end-column electrochemiluminescence detection. Electrophoresis 23, 3683–3691.

    Google Scholar 

  38. Yoon, S., Ban, E., and Yoo, Y. S. (2002) Direct monitoring of the expression of the green fluorescent protein–extracellular signal-regulated kinase 2 fusion protein in transfected cells using capillary electrophoresis with laser-induced fluorescence detection. J. Chromatogr. A 976, 87–93.

    CAS  PubMed  Google Scholar 

  39. Kiessig, S., Reissmann, J., Rascher, C., Kullertz, G., Fischer, A., and Thunecke, F. (2001) Application of a green fluorescent fusion protein to study protein-protein interactions by electrophoretic methods. Electrophoresis 22, 1428–1435.

    CAS  PubMed  Google Scholar 

  40. Zhang, Y. and Gomez, F. A. (2000) On-column derivatization and analysis of amino acids, peptides, and alkylamines by anhydrides using capillary electrophoresis. Electrophoresis 21, 3305–3310.

    CAS  PubMed  Google Scholar 

  41. Strong, R. A., Liu, H., Krull, I. S., Cho, B. -Y., and Cohen, S. A. (2000) Advantages and limitations of derivatization of peptides for improved performance and detectability in capillary isoelectric focusing (CIEF). J. Liq. Chromatogr. Relat. Technol. 23, 175–187.

    Google Scholar 

  42. Little, M. J., Paquette, D. M., Harvey, M. D., and Banks, P. R. (1997) Single-label fluorescent derivatization of peptides. Anal. Chim. Acta 339, 279–288.

    CAS  Google Scholar 

  43. Liu, H. J., Cho, B. Y., Krull, I. S., and Cohen, S. A. (2001) Homogeneous fluorescent derivatization of large proteins. J. Chromatogr. A 927, 77–89.

    CAS  PubMed  Google Scholar 

  44. Peterson, D. S., Rohr, T., Svec, F., and Frechet, J. M. J. [2002) Enzymatic microreactor-on-a-chip: protein mapping using trypsin immobilized on porous polymer monoliths molded in channels of microfluidic devices. Anal. Chem. 74, 4081–4088.

    CAS  PubMed  Google Scholar 

  45. Ghosal, S. (2002) Effect of analyte adsorption on the electroosmotic flow in microfluidic channels. Anal. Chem. 74, 771–775.

    CAS  PubMed  Google Scholar 

  46. Righetti, P. G., Gelfi, C., Verzola, B., and Castelleti, L. (2001) The state of the art of dynamic coatings. Electrophoresis 22, 603–611.

    CAS  PubMed  Google Scholar 

  47. Morand, M., Blaas, D., and Kenndler, E. (1997) Reduction of wall adsorption in capillary zone electrophoresis of a basic single-chain antibody fragment by a cationic polymeric buffer additive. J. Chromatogr. B 691, 192–196.

    CAS  Google Scholar 

  48. Ding, W. and Fritz, J. S. [1997) Separation of basic proteins and peptides by capillary electrophoresis using a cationic surfactant. J. High Resolut. Chromatogr. 20, 575–580.

    CAS  Google Scholar 

  49. Kasicka, V., Prusik, Z., Sazelova, P., Barth, T., Brynda, E., and Machova, L. (1997) Capillary zone electrophoresis with external radial electric field control of electroosmotic flow and its application to the separation of synthetic oligopeptides. J. Chromatogr. A 772, 221–230.

    CAS  Google Scholar 

  50. Cunliffe, J.M.; Baryla, N.E. and Lucy, C.A. (2002) Phospholipid bilayer coatings for the separation of proteins in capillary electrophoresis. Anal. Chem. 74, {776–783.}

    CAS  PubMed  Google Scholar 

  51. Emmer, A. and Roeraade, J. (2001) Wall deactivation with fluorosurfactants for capillary electrophoretic analysis of biomolecules. Electrophoresis 22, 660–665.

    CAS  PubMed  Google Scholar 

  52. Pacakova, V., Hubena, S., Ticha, M., and Madera, M. S. K. (2001) Effects of electrolyte modification and capillary coating on separation of glycoprotein isoforms by capillary electrophoresis. Electrophoresis 22, 459–463.

    CAS  PubMed  Google Scholar 

  53. Dou, Y. H., Bao, N., Xu, J. J., and Chen, H. Y. (2002) A dynamically modified microfluidic poly(dimethylsiloxane) chip with electrochemical detection for biological analysis. Electrophoresis 23, 3558–3566.

    CAS  PubMed  Google Scholar 

  54. Bao, J. J. (2000) Separation of proteins by capillary electrophoresis using an epoxy based hydrophilic coating. J. Liq. Chromaatogr. Rel. Technol. 23, 61–78.

    CAS  Google Scholar 

  55. Belenkii, B. G., Kassalainen, G. E., and Nasledov, D. G. (2000) Modified capillary electrophoresis system for peptide, protein and double-stranded DNA analysis. J. Chromatogr. A 879, 189–196.

    CAS  PubMed  Google Scholar 

  56. Cretich, M., Stastna, M., Chrambach, A., and Chiari, M. (2002) Decreased protein peak asymmetry and width due to static capillary coating with hydrophilic derivatives of polydimethylacrylamide. Electrophoresis 23, 2274–2278.

    CAS  PubMed  Google Scholar 

  57. Chiari, M., Cretich, M., Stastna, M., Radko, S. P., and Chrambach, A. (2001) Rapid capillary coating by epoxy-poly-(dimethylacrylamide): performance in capillary zone electrophoresis of protein and polystyrene carboxylate. Electrophoresis 22, 656–659.

    CAS  PubMed  Google Scholar 

  58. Leinweber, F. C., Stein, J., and Otto, M. (2001) Capillary zone electrophoresis of proteins with poly(2-hydroxyethyl methacrylate)-coated capillaries: fundamentals and applications. Fresenius J. Anal. Chem. 370, 781–788.

    CAS  PubMed  Google Scholar 

  59. Kleindienst, G., Huber, C. G., Gjerde, D. T., Yengoyan, L., and Bonn, G. K. (1998) Capillary electrophoresis of peptides and proteins in fused-silica capillaries coated with derivatized polystyrene nanoparticles. Electrophoresis 12, 262–269.

    Google Scholar 

  60. Zhang, J. and Horvath, C. (2003) Capillary electrophoresis of proteins in dextran-coated columns. Electrophoresis 24, 115–120.

    PubMed  Google Scholar 

  61. Le Potier, I., Taverna, M., Fattal, E., Benzaki, J., Chevalier, M., and Ferrier, D. (2001) Performance evaluation of capillary surface treatments for peptide mapping by capillary zone electrophoresis. Chromatographia 53, 563–570.

    Google Scholar 

  62. Tsai, S. W., Loughran, M., Hiratsuka, A., Yano, K., and Karube, I. (2003) Application of plasma-polymerized films for isoelectric focusing of proteins in a capillary electrophoresis chip. Analyst 128, 237–244.

    CAS  PubMed  Google Scholar 

  63. Gaudioso, J. and Craighead, H. G. (2002) Characterizing electroosmotic flow in microfluidic devices. J. Chromatogr. A 971, 249–253.

    CAS  PubMed  Google Scholar 

  64. Nilsson, H., Wiklund, M., Johansson, T., Hertz, H. M., and Nilsson, S. (2001) Microparticles for selective protein determination in capillary electrophoresis. Electrophoresis 22, 2384–2390.

    CAS  PubMed  Google Scholar 

  65. Kubo, K. and Hattori, A. (2001) Assessment of the capillary zone electrophoretic behavior of proteins in the presence of electroosmotic modifiers: protein-polyamine interaction studied using a polyacrylamide-coated capillary. Electrophoresis 22, 3389–3394.

    CAS  PubMed  Google Scholar 

  66. Tabuchi, M. and Baba, Y. (2001) A separation carrier in high-speed proteome analysis by capillary electrophoresis. Electrophoresis 22, 3449–3457.

    CAS  PubMed  Google Scholar 

  67. Macka, H., Johns, C., Grosse, A., and Haddad, P. R. (2001) New isoelectric buffers for capillary electrophoresis: N-carboxymethylated polyethyleneimine as a macromolecular isoelectric buffer. Analyst 126, 421–425.

    CAS  PubMed  Google Scholar 

  68. Tseng, W. L., Lin, Y. W., and Chang, H. T. (2002) Improved separation of microheterogeneities and isoforms of proteins by capillary electrophoresis using segmental filling with SDS and PEO in the background electrolyte. Anal. Chem. 74, 4828–4834.

    CAS  PubMed  Google Scholar 

  69. Mosulishvili, L. M., Barnov, V. A., Tsibakhashvili, N. Y., Engelhardt, H., and Beck, W. J. (2001) Enhancing detection sensitivity in capillary electrophoresis. J. Anal. Chem. 56, 512–514.

    CAS  Google Scholar 

  70. Wu, X. Z. and Pawliszyn, J. (2002) Whole-column imaging capillary electrophoresis of proteins with a short capillary. Electrophoresis 23, 542–549.

    CAS  PubMed  Google Scholar 

  71. Wu, X. Z., Sze, N. S. K., and Pawliszyn, J. (2001) Miniaturization of capillary isoelectric focusing. Electrophoresis 22, 3968–3971.

    CAS  PubMed  Google Scholar 

  72. Jin, L. J., Giordano, B. C., and Landers, J. P. (2001) Dynamic labeling during capillary or microchip electrophoresis for laser-induced fluorescence detection of protein-SDS complexes without pre- or postcolumn labeling. Anal. Chem. 23, 1090–1095.

    Google Scholar 

  73. McCorquodale, E. M. and Coyler, C. L. (2001) Indocyanine green as a noncovalent, pseudofluorogenic label for protein determination by capillary electrophoresis. Electrophoresis 22, 2403–2408.

    CAS  PubMed  Google Scholar 

  74. Sze, N. S. K., Huang, T. M., and Pawliszyn, J. (2002) Laser-induced fluorescence detection of non-covalently labeled protein in capillary isoelectric focusing. J. Sep. Sci. 25, 1119–1122.

    CAS  Google Scholar 

  75. Sowell, J., Agnew-Heard, K. A., Mason, J. C., Mama, C., Strekowski, L., and Patonay, G. (2001) Use of non-covalent labeling in illustrating ligand binding to human serum albumin via affinity capillary electrophoresis with near-infrared laser induced fluorescence detection. J. Chromatogr. B 755, 91–99.

    CAS  Google Scholar 

  76. Harvey, M. D., Bablekis, V., Banks, P. R., and Skinner, C. D. (2001) Utilization of the non-covalent fluorescent dye, NanoOrange, as a potential clinical diagnostic tool. nanomolar human serum albumin quantitation. J. Chromatogr. B 754, {345–356.}

    CAS  Google Scholar 

  77. Zhang, X. and Sweedler, J. V. (2001) Ultraviolet native fluorescence detection in capillary electrophoresis using a metal vapor NeCu laser. Anal. Chem. 73, 5620–5624

    CAS  PubMed  Google Scholar 

  78. Okerberg, E. and Shear, J. B. (2001) Attomole-level protein fingerprinting based on intrinsic peptide fluorescence. Anal. Chem. 73, 1610–1613.

    CAS  PubMed  Google Scholar 

  79. He, Y. and Geng, L. (2001) Phase-sensitive fluorescence lifetime detection in capillary electrophoresis. Anal. Chem. 73, 943–950.

    CAS  PubMed  Google Scholar 

  80. LeCaptain, D. J., Michel, M. A., and Van Orden, A. (2001) Characterization of DNA-protein complexes by capillary electrophoresis-single molecule fluorescence correlation spectroscopy. Analyst 126, 1279–1284.

    CAS  PubMed  Google Scholar 

  81. Le, X. C., Wang, Q. H., and Lam, M. T. (2002) Fluorescence polarization detection for affinity capillary electrophoresis. Electrophoresis 23, 903–908.

    CAS  PubMed  Google Scholar 

  82. Chabinyc, M. L., Chiu, D. T., McDonald, J. C., et al. (2001) An integrated fluorescence detection system in poly(dimethylsiloxane) for microfluidic applications. Anal. Chem. 73, 4491–4498.

    CAS  PubMed  Google Scholar 

  83. Sanders, J. C., Huang, Z. L., and Landers, J. P. (2001) Acousto-optical deflection-based whole channel scanning for microchip isoelectric focusing with laser-induced fluorescence detection. Lab on a Chip 1, 167–172.

    CAS  PubMed  Google Scholar 

  84. Wetterhall, M., Palmblad, M., Hakansson, P., Markides, K. E., and Bergquist, J. (2002) Rapid analysis of tryptically digested cerebrospinal fluid using capillary electrophoresis-electrospray ionization-fourier transform ion cyclotron resonance-mass spectrometry. J. Proteome Res. 1, 361–366.

    CAS  PubMed  Google Scholar 

  85. Tsybin, Y. O., Hakansson, P. W. M., Markides, K. E., and Bergquist, J. (2002) Capillary electrophoresis and electron capture dissociation Fourier transform ion cyclotron resonance mass spectrometry for peptide mixture and protein digest analysis. Eur. J. Mass Spectrom. 8, 389–395.

    CAS  Google Scholar 

  86. Huber, C. G., Premstaller, A., and Kleindienst, G. (1999) Evaluation of volatile eluents and electrolytes for high-performance liquid chromatography–electrospray\vvsp{-1} ionization mass spectrometry and capillary electrophoresis–electrospray ionization mass spectrometry of proteins: II. capillary electrophoresis. J. Chromatogr. A 849, 175–189.

    CAS  PubMed  Google Scholar 

  87. Lynen, F., Zhao, Y., Becu, C., Borremans, F., and Sandra, P. (1999) Considerations concerning interaction characterization of oligopeptide mixtures with vancomycin using affinity capillary electrophoresis-electrospray mass spectrometry. Electrophoresis 20, 2462–2474.

    CAS  PubMed  Google Scholar 

  88. Zhang, C. X., Xiang, F., Pasa-Tolic, L., Anderson, G. A., Veenstra, T. D., and Smith, R. D. (2000) Stepwise mobilization of focused proteins in capillary isoelectric focusing mass spectrometry. Anal. Chem. 72, 1462–1468.

    CAS  PubMed  Google Scholar 

  89. Tong, W., Link, A., Eng, J. K., and Yates III, J. R. (1999) Identification of proteins in complexes by solid-phase microextraction/multistep elution/capillary electrophoresis/tandem mass spectrometry. Anal. Chem. 71, 2270–2278.

    CAS  PubMed  Google Scholar 

  90. Li, J., Kelly, J. F., Chernushevich, I., Harrison, D. J., and Thibault, P. (2000) Separation and identification of peptides from gel-isolated membrane proteins using a microfabricated device for combined capillary electrophoresis/nanoelectrospray mass spectrometry. Anal. Chem. 72, 599–609.

    CAS  PubMed  Google Scholar 

  91. Liu, J., Tseng, K., Garcia, B., et al. (2001) Electrophoresis separation in open microchannels. a method for coupling electrophoresis with MALDI-MS. Anal. Chem. 73, 2147–2151.

    CAS  PubMed  Google Scholar 

  92. Hiraoka, A., Tominaga, I., and Hori, K. (2002) One-step capillary isoelectric focusing of the proteins in cerebrospinal fluid and serum of patients with neurological disorders. J. Chromatogr. A 961, 147–153.

    CAS  Google Scholar 

  93. Hashimoto, M., Tsugoagoshi, K. N. R., and Kondo, K. (1999) Chemiluminescence detection of heme proteins separated by capillary isoelectric focusing. J. Chromatogr. A 852, 597–601.

    CAS  PubMed  Google Scholar 

  94. Kautz, R. A., Lacey, M. E., Wolters, A. M., et al. (2001) Sample concentration and separation for nanoliter-volume NMR spectroscopy using capillary isotachophoresis. J. Am. Chem. Soc. 123, 3159–3160.

    CAS  PubMed  Google Scholar 

  95. Guijt, R. M., Baltussen, E., van der Steen, G., et al. (2001) Capillary electrophoresis with on-chip four-electrode capacitively coupled conductivity detection for application in bioanalysis. Electrophoresis 22, 2537–2541.

    CAS  PubMed  Google Scholar 

  96. Kuijt, J., van Teylingen, R., Nijbacker, T., Ariese, F., Brinkman, U. A. T., and Gooijer, C. (2001) Detection of nonderivatized peptides in capillary electrophoresis using quenched phosphorescence. Anal. Chem. 73, 5026–5029.

    CAS  PubMed  Google Scholar 

  97. Chen, J. Z. and Lee, C. S. (2002) On-line post-capillary affinity detection of immunoglobulin G for capillary zone electrophoresis. J. Chromatogr. B 768, 105–111.

    CAS  Google Scholar 

  98. Guijt-van Duijn, R. M., Frank, J., van Dedem, G. W., and Baltussen, E. (2000) Recent advances in affinity capillary electrophoresis. Electrophoresis 21, {3905–3918.}

    Google Scholar 

  99. Heegaard, N. H. H. and Kennedy, R. T. (1999) Identification, quantitation, and characterization of biomolecules by capillary electrophoretic analysis of binding interactions. Electrophoresis 20, 3122–3133. <ftskip0.4pc

    CAS  PubMed  Google Scholar 

  100. Tanaka, Y. and Terabe, S. (2002) Estimation of binding constants by capillary electrophoresis. J. Chromatogr. B 768, 81–92.

    CAS  Google Scholar 

  101. Lynen, F., Borremans, F., and Sandra, P. (2001) Practical evaluation of the influence of excessive sample concentration on the estimation of dissociation constants with affinity capillary electrophoresis. Electrophoresis 22, 1974–1978.

    CAS  PubMed  Google Scholar 

  102. Galbusera, C., Thachuk, M., De Lorenzi, E., and Chen, D. D. Y. (2002) Affinity capillary electrophoresis using a low-concentration additive with the consideration of relative mobilities. Anal. Chem. 74, 1903–1914.

    CAS  PubMed  Google Scholar 

  103. Heintz, J., Hernandez, M. and Gomez, F. A. (1999) Use of a partial-filling technique in affinity capillary electrophoresis for determining binding constants of ligands to receptors. J. Chromatogr. A 840, 261–280.

    CAS  PubMed  Google Scholar 

  104. Kiessig, S., Bang, H., and Thunecke, F. (1999) Interaction of cyclophilin and cyclosporins monitored by affinity capillary electrophoresis. J. Chromatogr. A 853, 469–477.

    CAS  PubMed  Google Scholar 

  105. Hafner, F. T., Kautz, R. A., Iverson, B. L., Tim, R. C., and Karger, B. L. (2000) Noncompetitive immunoassay of small analytes at the femtomolar level by affinity probe capillary electrophoresis: direct analysis of digoxin using a uniform-labeled scFv immunoreagent. Anal. Chem. 72, 5779–5786.

    CAS  PubMed  Google Scholar 

  106. Foulds, G. J. and Etzkorn, F. A. (1999) DNA-binding affinities of MyoD and E47 homo- and hetero-dimers by capillary electrophoresis mobility shift assay. J. Chromatogr. A 862, 231–236.

    CAS  PubMed  Google Scholar 

  107. Kim, H. S., Austin, J., and Hage, D. S. (2002) Identification of drug-binding sites on human serum albumin using affinity capillary electrophoresis and chemically modified proteins as buffer additives. Electrophoresis 23, 956–963.

    CAS  PubMed  Google Scholar 

  108. Bean, S. R. and Lookhart, G. L. (1999) Sodium dodecyl sulfate capillary electrophoresis of wheat proteins. 1. uncoated capillaries. J. Agric. Food Chem. 47, 4246–4255.

    CAS  PubMed  Google Scholar 

  109. Hu, S., Zhang, Z., Cook, L. M., Carpenter, E. J., and Dovichi, N. J. (2000) Separation of proteins by sodium dodecylsulfate capillary electrophoresis in hydroxypropylcellulose sieving matrix with laser-induced fluorescence detection. J. Chromatogr. A 894, 291–296.

    CAS  PubMed  Google Scholar 

  110. Bousse, L., Mouradian, S., Minalla, A., Yee, H., Williams, K., and Dubrow, R. (2001) Protein sizing on a microchip. Anal. Chem. 73, 1207–1212.

    CAS  PubMed  Google Scholar 

  111. Hu, S., Ye, Y. L., Surh, G., Clark, J. I., and Dovichi, N. J. (2002) Analysis of proteins by capillary SDS-DALT electrophoresis with laser-induced fluorescence detection. LC-GC 15, 166.

    Google Scholar 

  112. Hu, S., Zhang, L., Cook, L. M., and Dovichi, N. J. (2001) Capillary sodium dodecyl sulfate-DALT electrophoresis of proteins in a single human cancer cell. Electrophoresis 22, 3677–3682.

    CAS  PubMed  Google Scholar 

  113. Shen, Y., Xiang, F., Veenstra, T. D., Fung, E. N. and Smith, R. D. (1999) High-resolution capillary isoelectric focusing of complex protein mixtures from lysates of microorganisms. Anal. Chem. 71, 5348–5353.

    CAS  PubMed  Google Scholar 

  114. König, S. and Welsch, T. (2000) Moderation of the electroosmotic flow in capillary electrophoresis by chemical modification of the capillary surface with tentacle-like oligourethanes. J. Chromatogr. A 894, 79–88.

    PubMed  Google Scholar 

  115. Shimura, K., Wang, Z., Matsumoto, H., and Kasai, K. -I. (2000) Synthetic oligopeptides as isoelectric point markers for capillary isoelectric focusing with ultraviolet absorption detection. Electrophoresis 21, 603–610.

    CAS  PubMed  Google Scholar 

  116. Mao, Q. and Pawliszyn, J. (1999) Capillary isoelectric focusing with whole column imaging detection for analysis of proteins and peptides. J. Biochem. Biophys. Methods 39, 93–110.

    CAS  PubMed  Google Scholar 

  117. Wen, J., Lin, Y., Xiang, F., Matson, D. W., Udseth, H. R., and Smith, R. D. (2000) Microfabricated isoelectric focusing device for direct electrospray ionization-mass spectrometry. Electrophoresis 21, 191–197.

    CAS  PubMed  Google Scholar 

  118. Tran, N. T., Taverna, M., Chevalier, M., and Ferrier, D. (2000) One-step capillary isoelectric focusing for the separation of the recombinant human immunodeficiency virus envelope glycoprotein glycoforms. J. Chromatogr. A 866, 121–135.

    CAS  PubMed  Google Scholar 

  119. Lopez-Soto-Yarritu, P., Diez-Masa, J. C., Cifuentes, A., and De Frutos, M. (2002) Improved capillary isoelectric focusing method for recombinant erythropoietin analysis. J. Chromatogr. A 968, 221–228.

    CAS  PubMed  Google Scholar 

  120. Ladsun, A. M., Kurumbail, R. R., Leimgruber, N. K., and Rathore, A. S. (2001) Validatibility of a capillary isoelectric focusing method for impurity quantitation. J. Chromatogr. A 917, 147–158.

    Google Scholar 

  121. Mohan, D. and Lee, C. S. (2002) On-line coupling of capillary isoelectric focusing with transient isotachophoresis-zone electrophoresis: a two-dimensional separation system for proteomics. Electrophoresis 23, 3160–3167.

    CAS  PubMed  Google Scholar 

  122. Sheng, L. and Pawliszyn, J. (2002) Comprehensive two dimensional separation based on coupling micellar electrokinetic chromatography with capillary isoelectric focusing. Analyst 127, 1159–1163.

    CAS  PubMed  Google Scholar 

  123. Issaq, H. J., Chan, K. C., Janini, G. M., and Muschik, G. M. (1999) A simple two-dimensional high performance liquid chromatography/high performance capillary electrophoresis set-up for the separation of complex mixtures. Electrophoresis 20, 1533–1537.

    CAS  PubMed  Google Scholar 

  124. Wall, D. B., Kachman, M. T., Gong, S., et al. (2000) Isoelectric focusing nonporous RP HPLC: a two-dimensional liquid-phase separation method for mapping of cellular proteins with identification using MALDI-TOF mass spectrometry. Anal. Chem. 72, 1099–1111.

    CAS  PubMed  Google Scholar 

  125. Tragas, C. and Pawliszyn, J. (2000) On-line coupling of high performance gel filtration chromatography with imaged capillary isoelectric focusing using a membrane interface. Electrophoresis 21, 227–237.

    CAS  PubMed  Google Scholar 

  126. Chen, X. X., Wu, H. K., Mao, C. D., and Whitesides, G. M. (2002) A prototype two-dimensional capillary electrophoresis system fabricated in poly(dimethylsiloxane). Anal. Chem. 74, 1772–1778.

    CAS  PubMed  Google Scholar 

  127. Herr, A. E., Molho, J. I., Drouvalakis, K. A., et al. (2003) On-chip coupling of isoelectric focusing and free solution electrophoresis for multidimensional separations. Anal. Chem. 75, 1180–1187.

    CAS  PubMed  Google Scholar 

  128. Claeys, R., Groven, C., and Gorus, F. K. (2001) Capillary zone electrophoresis of proteins in body Fluids: comparison of capillary and agarose gel electrophoresis. Clin. Chem. 47, 967–970.

    CAS  PubMed  Google Scholar 

  129. Bossuyt, X., Claeys, R., Bogaert, G., et al. (2001) Reference values for the five electrophoretic serum protein fractions in Caucasian children by capillary zone electrophoresis. Clin. Chem. Lab. 39, 970–972.

    CAS  Google Scholar 

  130. Le Bricon, T., Launay, E., Houze, P., Bengoufa, D., Bousquet, B., and Gourmel, B. (2002) Determination of poorly separated monoclonal serum proteins by capillary zone electrophoresis. J. Chromatogr. B 775, 63–70.

    Google Scholar 

  131. Jia, Z. J., Ramstad, T., and Zhong, M. (2002) Determination of protein–drug binding constants by pressure-assisted capillary electrophoresis (PACE)/frontal analysis (FA). J. Pharm. Biomed. Anal. 30, 405–413.

    CAS  PubMed  Google Scholar 

  132. Shimura, K., Hoshino, M., Kamiya, K., et al. (2002) Immunoassay of serum α1-antitrypsin by affinity-probe capillary isoelectric focusing using a fluorescence-labeled recombinant antibody fragment. Electrophoresis 23, 909–917.

    CAS  PubMed  Google Scholar 

  133. Varesio, E., Rudaz, S., Krause, K. H., and Veuthey, J. L. (2002) Nanoscale liquid chromatography and capillary electrophoresis coupled to electrospray mass spectrometry for the detection of amyloid- peptide related to Alzheimer. J. Chromatogr. A 974, 135–142.

    CAS  PubMed  Google Scholar 

  134. Clarke, N. J. and Naylor, S. (2002) Capillary isoelectric focusing-mass spectrometry: analysis of protein mixtures from human body fluids. Biomed. Chromatogr. 16, 287–297.

    CAS  PubMed  Google Scholar 

  135. Moini, M., Demars, S. M., and Huang, H. L. (2002) Analysis of carbonic anhydrase in human red blood cells using capillary electrophoresis/electrospray ionization-mass spectrometry. Anal. Chem. 74, 3772–3776.

    CAS  PubMed  Google Scholar 

  136. Kolios, G., Bairaktari, E., Tsolas, O. and Seferiadis, K. (2001) Routine differential diagnosis of proteinurias by capillary electrophoresis. Clin. Chem. Lab. Med. 39, 784–788.

    CAS  PubMed  Google Scholar 

  137. Porcel, J. M., Vives, M., Esquerda, A., and Rivas, M. C. (2001) Pleural protein capillary electrophoresis for the separation of transudates and exudates. Clin. Chem. 47, 975–976.

    CAS  PubMed  Google Scholar 

  138. Hu, S., Jiang, J., Cook, L. M., et al. (2002) Capillary sodium dodecyl sulfate-DALT electrophoresis with laser-induced fluorescence detection for size-based analysis of proteins in human colon cancer cells. Electrophoresis 23, 3136–3142.

    CAS  PubMed  Google Scholar 

  139. Jenkins, M. A. and Ratnaike, S. (1999) Five unusual serum protein presentations found by capillary electrophoresis in the clinical laboratory. J. Biochem. Biophys. Methods 41, 31–47.

    CAS  PubMed  Google Scholar 

  140. Hiraoka, A., Tominaga, I., and Hori, K. (2000) Sodium dodecylsulfate capillary gel electrophoretic measurement of the concentration ratios of albumin and alpha2-macroglobulin in cerebrospinal fluid and serum of patients with neurological disorders. J. Chromatogr. A 895, 339–344.

    CAS  PubMed  Google Scholar 

  141. Frazier, R.A. (2001) Recent advances in capillary electrophoresis methods for food analysis. Electrophoresis 22, 4197–4206.

    CAS  PubMed  Google Scholar 

  142. Recio, I., Ramos, M., and Lopez-Fandino, R. (2001) Capillary electrophoresis for the analysis of food proteins of animal origin. Electrophoresis 22, 1489–1502.

    CAS  PubMed  Google Scholar 

  143. Zhu, J. and Khan, K. (2001) Separation and quantification of HMW glutenin subunits by capillary electrophoresis. Cereal Chem. 78, 737–742.

    CAS  Google Scholar 

  144. Siriamornpun, S., Wootton, M., Cox, J. M., Bekes, F., and Wrigley, C. W. A. (2001) Capillary electrophoresis of wheat gliadin proteins and its potential for wheat varietal identification using pattern matching software. Aust. J. Agr. Res. 52, 839–843.

    CAS  Google Scholar 

  145. Scholz, E., Ganzler, K., Gergely, S., and Salgo, A. (2002) Use of capillary electrophoresis to monitor wheat maturation. Chromatographia 56, S127–S130.

    Google Scholar 

  146. Strickland, M., Johnson, M. E., and Broadbent, J. R. (2001) Qualitative and quantitative analysis of proteins and peptides in milk products by capillary electrophoresis. Electrophoresis 22, 1510–1517.

    CAS  PubMed  Google Scholar 

  147. Miralles, B., Rothbauer, V., Manso, M. A., Amigo, L., Krause, I., and Ramos, M. (2001) Improved method for the simultaneous determination of whey proteins, caseins and para-casein in milk and dairy products by capillary electrophoresis. J. Chromatogr. A 915, 225–230.

    CAS  Google Scholar 

  148. De Block, J., Merchiers, M., Mortiers, L., et al. (2003) Monitoring nutritional quality of milk powders: capillary electrophoresis of the whey protein fraction compared with other methods. Int. Diary J. 13, 87–94.

    Google Scholar 

  149. Fayle, S. E., Healy, J. P., Brown, P. A., Reid, E. A., Gerrard, J. A., and Ames, J. M. (2001) Novel approaches to the analysis of the Maillard reaction of proteins. Electrophoresis 22, 1518–1525.

    CAS  PubMed  Google Scholar 

  150. Guyomarch, F., Warin, F., Muir, D. D., and Leaver, J. (2000) Lactosylation of milk proteins during the manufacture and storage of skim milk powders. Int. Diary J. 10, 863–872.

    CAS  Google Scholar 

  151. Bertoldo Pacheco, M. T., Amaya-Farfan, J., and Sgarbieri, V. (2002) Partial characterization of a whey protein concentrate and its enzyme hydrolysates. J. Food Biochem. 26, 327–338.

    Google Scholar 

  152. Civardi, G., Curadi, M. C., Orlandi, M., Cattaneo, T. M. P., and Giangiacomo, R. (2002) Capillary electrophoresis (CE) applied to analysis of mare’s milk. Milchwissenschaft 82, 1240–1245.

    Google Scholar 

  153. Molina, E., Ramos, M., and Amigo, L. (2002) Characterisation of the casein fraction of Iberico cheese by electrophoretic techniques. J. Sci. Food Agric. 82, 1240–1245.

    CAS  Google Scholar 

  154. Day, L. and Brown, H. (2001) Detection of mechanically recovered chicken meat using capillary gel electrophoresis. Meat Sci. 58, 31–37.

    CAS  Google Scholar 

  155. Martin A., Cordoba J. J., Rodriguez M. M., Nunez F., and Asensio, M. A. (2001) Evaluation of microbial proteolysis in meat products by capillary electrophoresis. J. Appl. Microbiol. 90, 163–171.

    CAS  PubMed  Google Scholar 

  156. Larrain, M. A., Abugoch, L., Quitral, V., Vinagre, J., and Segovia, C. (2002) Capillary zone electrophoresis as a method for identification of golden kinglip (Genypterus blacodes) species during frozen storage. Food Chem. 76, 377–384.

    CAS  Google Scholar 

  157. Rodriguez-Delgado, M. A., Malovana, S., Montelongo, F. J., and Cifuentes (2002) Fast analysis of proteins in wines by capillary gel electrophoresis. Eur. Food Res. Technol. 214, 536–540.

    CAS  Google Scholar 

  158. Mazhar, H., Basha, S. M., and Lu, J. (2002) Variation in berry protein composition of muscadine cultivars. Am. J. Enol. Viticul. 53, 87–91.

    CAS  Google Scholar 

  159. Righetti, P. G. (2001) Capillary-electrophoretic analysis of proteins and peptides of biomedical and pharmacological interest. Biopharm. Drug Disp. 22, 337–351.

    CAS  Google Scholar 

  160. Gawron, A. J., Martin, R. S., and Lunte, S. (2001) Microchip electrophoretic separation systems for biomedical and pharmaceutical analysis. Eur. J. Pharm. Sci. 14, 1–12.

    CAS  PubMed  Google Scholar 

  161. Hwang, K. -H., Lee, K. W., Kim, C. S., Han, K., Chung, Y. -B. and Moon, D. -C. (2001) Determination of recombinant human epidermal growth factor (rhEGF) in a pharmaceutical preparation by capillary electrophoresis. Arch. Pharm. Res. 24, 601–606.

    CAS  PubMed  Google Scholar 

  162. Eriksson, H. J. C., Somsen, G. W., Hinrichs, W. L. J., Frijlink, H. W., and de Jong, G. J. (2001) Characterization of human placental alkaline phosphatase by activity and protein assays, capillary electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. J. Chromatogr. B 755, 311–319.

    CAS  Google Scholar 

  163. Eriksson, H. J. C., Wijngaard, M., Hinrichs, W. L. J., Frijlink, H. W., Somsen, G. W., and De Jong, G. J. (2003) Potential of capillary electrophoresis for the monitoring of the stability of placental alkaline phosphatase. J. Pharm. Biomed. 31, 351–357.

    CAS  Google Scholar 

  164. Jensen, P. K., Pasa-Tolic, L., Anderson, G. A., et al. (1999) Probing proteomes using capillary isoelectric focusing-electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry. Anal. Chem. 71, 2076–2084.

    CAS  PubMed  Google Scholar 

  165. Michels, D. A., Hu, S., Schoenherr, R. M., Eggerston, M. J., and Dovichi, N. J. (2002) Fully automated two-dimensional capillary electrophoresis for high sensitivity protein analysis. Mol. Cell. Proteomics 1, 69–74.

    CAS  PubMed  Google Scholar 

  166. Li, J. J., Tremblay, T. L., Wang, C., Attiya, S., Harrison, D. J., and Thibault, P. (2001) Integrated system for high-throughput protein identification using a microfabricated device coupled to capillary electrophoresis/nanoelectrospray mass spectrometry. Proetomics 1, 975–986.

    CAS  Google Scholar 

  167. Wu, X. Z., Huang, T. M., Mullett, W. M., and Yeung, J. M. (2001) Determination of isoelectric point and investigation of immunoreaction in peanut allergenic proteins-rabbit IgG antibody system by whole-column imaged capillary isoelectric focusing. J. Microcol. Sep. 13, 322–326.

    CAS  Google Scholar 

  168. Viskari, P. J., Kinkade, C. S., and Colyer, C. L. (2001) Determination of phycobiliproteins by capillary electrophoresis with laser-induced fluorescence detection. Electrophoresis 22, 2327–2335.

    CAS  PubMed  Google Scholar 

  169. Viskari, P. J. and Colyer, C. L. (2002) Separation and quantitation of phycobiliproteins using phytic acid in capillary electrophoresis with laser-induced fluorescence detection. J. Chromatogr. A. 972, 269–276.

    CAS  PubMed  Google Scholar 

  170. Deterding, L. J., Cutalo, J. M., Khaledi, M., and Tomer, K. B. (2002) Separation and characterization of human high-density apolipoproteins using a nonaqueous modifier in capillary electrophoresis-mass spectrometry. Electrophoresis 23, 2296–2305.

    CAS  PubMed  Google Scholar 

  171. Presley, A. D., Poe, B. G., and Arriaga, E. A. (2002) Separation and detection of fluorescently-labeled mitochondrial proteins by capillary electrophoresis with laser-induced fluorescence detection. Abstr. Papers ACS 223, 259.

    Google Scholar 

  172. Galli, A., Ghezzi, L., Raspi, G., Secco, F., and Spinetti, M. (2002) Detection of heterologous bovine pancreatic trypsin inhibitor by capillary zone electrophoresis. Polyhedron 21, 1405–1410.

    CAS  Google Scholar 

  173. Nicholas, K., Forney, C. F., and Paulson, A. T. (2002) A rapid capillary gel electrophoresis method for the quantitative determination of RuBisCo in spinach. Phytochem. Anal. 13, 39–44.

    CAS  PubMed  Google Scholar 

  174. Tseng, W. L., Chiu, T. C., Weng, J. M., and Chang, H. T.(2001) Analysis of albumins, using albumin blue 580, by capillary electrophoresis and laser-induced fluorescence. J. Liq. Chromatogr. 24, 2971–2982.

    CAS  Google Scholar 

  175. Murakami, Y., Morita, T., Kanekiyo, T., and Tamiya, E. (2001) On-chip capillary electrophoresis for alkaline phosphatase testing. Biosens. Bioelectron. 16, {1009–1014.}

    CAS  PubMed  Google Scholar 

  176. Soundararajan, S., Guariglia, L., Sydor, W., and Mercorelli, S. (2001) Analysis of an IgG4 monoclonal antibody by protein lab-on-chip technology, SDS-capillary gel electrophoresis and SDS-polyacrylamide gel electrophoresis. Abstr. Papers ACS 222, 16.

    Google Scholar 

  177. Starkey, D. E., Han, A., Abdelaziez, Y., et al. (2001) Fluorogenic assays for β-glucuronidase and mitogen activated protein kinase using microchip capillary electrophoresis (µ CE). Abstr. Papers ACS 222, 70.

    Google Scholar 

  178. Schrum, D. P. and Worthington, M. (2001) Analysis of amyloid β-A4-related peptides, amyloid protein fibrils, and their interactions by capillary electrophoresis. Abstr. Papers ACS 221, 720.

    Google Scholar 

  179. Liu, C. M. and Tzeng, Y. M. (2001) Quantitative analysis of parasporal crystal protein from Bacillus thuringiensis by capillary electrophoresis. J. Food Drug Anal. 9, 79–83.

    CAS  Google Scholar 

  180. Ledger, R., Tucker, I. G., and Walker, G. F. (2002) Quantitative capillary electrophoresis assay for the proteolytic stability of luteinizing hormone-releasing hormones. J. Chromatogr. B 769, 235–242.

    CAS  Google Scholar 

  181. Dai, H. J., Parker, C. N., and Bao, J. J. (2002) Characterization and inhibition study of MurA enzyme by capillary electrophoresis. J. Chromatogr. B 766, {123–132.}

    CAS  Google Scholar 

  182. Sowell, J., Parihar, R., and Patonay, G. (2001) Capillary electrophoresis-based immunoassay for insulin antibodies with near-infrared laser induced fluorescence detection. J. Chromatogr. B 752, 1–8.

    CAS  Google Scholar 

  183. Chiti, F., De Lorenzi, E., Grossi, S., et al. (2001) A partially structured species of UPbeta2-microglobulin is significantly populated under physiological conditions and involved in fibrillogenesis. J. Biol. Chem. 276, 46714–46721.

    CAS  PubMed  Google Scholar 

  184. Heegaard, N. H. H., Sen, J. W., Kaarsholm, N. C., and Nissen, M. H. (2001) Conformational intermediate of the amyloidogenic protein UPbeta2-microglobulin at neutral pH. J. Biol. Chem. 276, 32657–32662.

    CAS  PubMed  Google Scholar 

  185. Gu, X. L., Whipple-Vanpatter, G., O‘Dwyer, M., and Zeece, M. (2001) Capillary electrophoretic analysis of UPmu- and m-calpain using fluorescently labeled casein substrates. Electrophoresis 22, 2336–2342.

    CAS  PubMed  Google Scholar 

  186. Keren, D. F., Gulbranson, R., Carey, J. L., and Krauss, J. C. (2001) 2-Mercaptoethanol treatment improves measurement of an IgMUPkappa M-protein by capillary electrophoresis. Clin. Chem. 47, 1326–1327.

    CAS  PubMed  Google Scholar 

  187. Hu, S., Lee, R., Zhang, Z., Krylov, S. N. and Dovichi, N. J. (2001) Protein analysis of an individual Caenorhabditis elegans single-cell embryo by capillary electrophoresis. J. Chromatogr. B 752, 307–310.

    CAS  Google Scholar 

  188. Li, H., Wu, H. Y., Wang, Y., Sims, C. E., and Allbritton, N. L. (2001) Improved capillary electrophoresis conditions for the separation of kinase substrates by the laser micropipet system. J. Chromatogr. B 757, 79–88.

    CAS  Google Scholar 

  189. Negin, R. S. and Carbeck, J. D. (2002) Measurement of electrostatic interactions in protein folding with the use of protein charge ladders. J. Am. Chem. Soc. 124, 2911–2916.

    CAS  PubMed  Google Scholar 

  190. Hurlbert, J. and Preston, J. F. (2002) Differences in the solution structures of the parallel beta-helical pectate lyases as determined by limited proteolysis. Biochim. Biophys. Acta 1599, 9–20.

    PubMed  Google Scholar 

  191. Stellwagen Earle, O. E. and Righetti, P. G. (2002) Salt-promoted protein folding, preferential binding, or electrostatic screening? Proteins 49, 147–153.

    CAS  PubMed  Google Scholar 

  192. Fei, Y., Wei, L., Gao, S., et al. (2001) Isolation, purification and characterization of secondary structure of antifreeze protein from Ammopiptanthus mongolicus. Chin. Sci. Bulletin 46, 495–498.

    CAS  Google Scholar 

  193. De Lorenzi, E., Grossi, S., Massolini, G., et al. (2002) Capillary electrophoresis investigation of a partially unfolded conformation of beta2-microglobulin. Electrophoresis 23, 918–925.

    Google Scholar 

  194. Rochu, D. and Maasson, P. (2002) Multiple advantages of capillary zone electrophoresis for exploring protein conformational stability. Electrophoresis 23, 189–202.

    CAS  PubMed  Google Scholar 

  195. Righetti, P. G. and Verzola, B. (2001) Folding/unfolding/refolding of proteins: present methodologies in comparison with capillary zone electrophoresis. Electrophoresis 22, 2359–2374.

    CAS  PubMed  Google Scholar 

  196. Uegaki, K., Taga, A., Akada, Y., Suzuki, S., and Honda, S. (2002) Simultaneous estimation of the association constants of glycoprotein glycoforms to a common protein by capillary electrophoresis. Anal. Biochem. 309, 269–278.

    CAS  PubMed  Google Scholar 

  197. Hattori, T., Kimura, K., Seyrek, E., and Dubin, P. L. (2001) Binding of bovine serum albumin to heparin determined by turbidimetric titration and frontal analysis continuous capillary electrophoresis. Anal. Biochem. 295, 158–167.

    CAS  PubMed  Google Scholar 

  198. Shibukawa, A., Ishizawa, N., Kimura, T., et al. (2002) Plasma protein binding study of oxybutynin by high-performance frontal analysis. J. Chromatogr. B 768, 177–188.

    CAS  Google Scholar 

  199. Onishi, T., Mohamed, N. A. L., Shibukawa, A., et al. (2002) Frontal analysis of drug–plasma lipoprotein binding using capillary electrophoresis. J. Pharm. Biomed. Anal. 27, 607–614.

    Google Scholar 

  200. Sowell, J., Mason, J. C., Strekowski, L. and Patonay, G. (2001) Binding constant determination of drugs toward subdomain IIIA of human serum albumin by near-infrared dye-displacement capillary electrophoresis. Electrophoresis 22, 2512–2517.

    CAS  PubMed  Google Scholar 

  201. Zhang, W. B., Zhang, L. H., Ping, G. C., Zhang, Y. K., and Kettrup, A. (2002) Study on the multiple sites binding of human serum albumin and porphyrin by affinity capillary electrophoresis. J. Chromatogr. B 768, 211–214.

    CAS  Google Scholar 

  202. Ding, Y. S., Lin, B. C., and Huie, C. W. (2001) Binding studies of porphyrins to human serum albumin using affinity capillary electrophoresis. Electrophoresis 22, 2210–2216.

    CAS  PubMed  Google Scholar 

  203. Rabiller-Baudry, M. and Chaufer, B. (2001) Specific adsorption of phosphate ions on proteins evidenced by capillary electrophoresis and reversed-phase high-performance liquid chromatography. J. Chromatogr. B 753, 67–77.

    CAS  Google Scholar 

  204. Kuroda, Y., Kita, Y., Shibukawa, A., and Nakagawa, T. (2001) Role of biantennary glycans and genetic variants of human alpha1-acid glycoprotein in enantioselective binding of basic drugs as studied by high performance frontal analysis/capillary electrophoresis. Pharm. Res. 18, 389–393.

    CAS  PubMed  Google Scholar 

  205. He, X. Y., Xiao, H. B., Liang, X. M., and Lin, B. C. (2002) Quantitative evaluation of the interaction between pUC19DNA and ovalbumin by capillary zone electrophoresis. J. Sep. Sci. 25, 711–714.

    CAS  Google Scholar 

  206. Purcell, M., Neault, J. F., Malonga, H., Arakawa, H., and Tajmir-Riahi, H. A. C. (2001) Interaction of human serum albumin with oxovanadium ions studied by FT-IR spectroscopy and gel and capillary electrophoresis. Can. J. Chem. 79, 1415–1421.

    CAS  Google Scholar 

  207. Liu, T., Li, J. D., Zeng, R., Shao, X. X., Wang, K. Y., and Xia, Q. C. (2001) Capillary electrophoresis-electrospray mass spectrometry for the characterization of high-mannose-type N-glycosylation and differential oxidation in glycoproteins by charge reversal and protease/glycosidase digestion. Anal. Chem. 73, 5875–5885.

    CAS  PubMed  Google Scholar 

  208. Grady, J. K., Zang, J., Laue, T. M., Arosio, P., and Chasteen, N. D. (2002) Characterization of the H- and L-subunit ratios of ferritins by sodium dodecyl sulfate–capillary gel electrophoresis. Anal. Biochem. 302, 263–268.

    CAS  PubMed  Google Scholar 

  209. Lee, H. G., Chang, S., and Fritsche, E. (2002) Rational approach to quantitative sodium dodecyl sulfate capillary gel electrophoresis of monoclonal antibodies. J. Chromatogr. A 947, 143–149.

    CAS  PubMed  Google Scholar 

  210. Page, J. S., Rubakhin, S. S., and Sweedler, J. V. (2002) Single-neuron analysis using CE combined with MALDI MS and radionuclide detection. Anal. Chem. 74, 497–503.

    CAS  PubMed  Google Scholar 

  211. Mohan, D. and Lee, C. S. (2002) On-line coupling of capillary isoelectric focusing with transient isotachophoresis-zone electrophoresis: a two-dimensional separation system for proteomics. Electrophoresis 23, 3160–3167.

    CAS  PubMed  Google Scholar 

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  1. Institute of Analytical Chemistry and Radiochemistry, Leopold-Franzens University, Innsbruck, Austria

    Christian W. Huck & Günther K. Bonn

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  1. HelmholtzZentrum München German Research Center for Environmental Health, Neuherberg, Germany

    Philippe Schmitt-Kopplin

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Huck, C.W., Bonn, G.K. (2008). Analysis of Proteins by Capillary Electrophoresis. In: Schmitt-Kopplin, P. (eds) Capillary Electrophoresis. Methods In Molecular Biology™, vol 384. Humana Press. https://doi.org/10.1007/978-1-59745-376-9_20

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