Summary
Microchip capillary electrophoresis (MCE) is gaining popularity due to the developments of simple microfabrication methods under nonstringent laboratory conditions. Moreover, the low material and production costs of polymer-based microchips have further stimulated advances in the applications of MCE in various fields, including clinical analysis, drug screening, biomarker identification, and biosensing. In this chapter, a simple and robust protocol for fabrication of microchips for lab-on-chip testing and microchip electrophoresis is described. The microchips are hybrid poly(dimethylsiloxane) (PDMS)/glass microchips, which are produced by a combination of photolithography and micromolding processes. This type of microchip has been used in a wide range of analyses.
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Acknowledgments
S.F.Y. Li acknowledges financial support from MOE (AcRF R-143-000-293-112), A-STAR (SERC-PSF 052 101 0044 and SBIC 009/2005), and EWI (MEWR C651/06/144).
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Tay, E.T.T., Law, W.S., Li, S.F.Y., Kricka, L.J. (2009). Microchip Capillary Electrophoresis. In: Bilitewski, U. (eds) Microchip Methods in Diagnostics. Methods in Molecular Biology™, vol 509. Humana Press. https://doi.org/10.1007/978-1-59745-372-1_11
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DOI: https://doi.org/10.1007/978-1-59745-372-1_11
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