Abstract
Budding yeast Saccharomyces cerevisiae is widely used to study mitochondrial biogenesis and function. We review some basic properties that make yeast an ideal model organism to investigate various aspects of mitochondrial biology. We discuss genetic features of commonly used yeast strains that are important for mitochondrial studies. Furthermore, this chapter provides protocols describing yeast culture conditions and procedures for isolation and purification of mitochondria.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Sherman, F. (1991) Getting started with yeast. Methods Enzymol. 194, 3–21.
Goffeau, A., Barrell, B. G., Bussey, H., et al. (1996) Life with 6000 genes. Science 274, 546–552.
Giaever, G., Chu, A. M., Ni, L., et al. (2002) Functional profiling of the Saccharomyces cerevisiae genome. Nature 418, 387–391.
Huh, W. K., Falvo, J. V., Gerke, L. C., et al. (2003) Global analysis of protein localization in budding yeast. Nature 425, 686–691.
Ghaemmaghami, S., Huh, W. K., Bower, K., et al. (2003) Global analysis of protein expression in yeast. Nature 425, 737–741.
Mnaimneh, S., Davierwala, A. P., Haynes, J., et al. (2004) Exploration of essential gene functions via titratable promoter alleles. Cell 118, 31–44.
Martin, A.C. and Drubin, D. G. (2003) Impact of genome-wide functional analyses on cell biology research. Curr. Opin. Cell Biol. 15, 6–13.
Christie, K. R., Weng, S., Balakrishnan, R., et al. (2004) Saccharomyces Genome Database (SGD) provides tools to identify and analyze sequences from Saccharomyces cerevisiae and related sequences from other organisms. Nucleic Acids Res. 32, D311–D314.
Güldener, U., Münsterkötter, M., Kastenmüller, G., et al. (2005) CYGD: the Comprehensive Yeast Genome Database. Nucleic Acids Res. 33, D364–D368.
Gancedo, J.M. (1998) Yeast carbon catabolite repression. Microbiol. Mol. Biol. Rev. 62, 334–361.
Stevens, B. (1981) Mitochondrial structure, in The Molecular Biology of the Yeast Saccharomyces: Life Cycle and Inheritance (Strathern, E.W., Jones, E.W., and Broach, J.R., eds.), Cold Spring Harbor Press, Cold Spring Harbor, NY, pp. 471–504.
Pon, L. and Schatz, G. (1991) Biogenesis of yeast mitochondria, in The Molecular Biology of the Yeast Saccharomyces: Genome Dynamics, Protein Synthesis, and Energetics (Broach, J.R., Pringle, J.R., and Jones, E.W., eds.), Cold Spring Harbor Press, Cold Spring Harbor, NY, pp. 333–406.
Egner, A., Jakobs, S., and Hell, S. W. (2002) Fast 100 nm resolution 3D-microscope reveals structural plasticity of mitochondria in live yeast. Proc. Natl. Acad. Sci. U. S. A. 99, 3370–3375.
Visser, W., van Spronsen, E. A., Nanninga, N., Pronk, J. T., Gijs Kuenen, J., and van Dijken, J.P. (1995) Effects of growth conditions on mitochondrial morphology in Saccharomyces cerevisiae. Antonie Van Leeuwenhoek 67, 243–253.
de Zamaroczy, M. and Bernardi, G. (1985) Sequence organization of the mitochondrial genome of yeast-a review. Gene 37, 1–17.
Dimmer, K. S., Fritz, S., Fuchs, F., et al. (2002) Genetic basis of mitochondrial function and morphology in Saccharomyces cerevisiae. Mol. Biol. Cell 13, 847–853.
Tzagoloff, A. and Dieckmann, C. L. (1990) PET genes of Saccharomyces cerevisiae. Microbiol. Rev. 54, 211–225.
Sickmann, A., Reinders, J., Wagner, Y., et al. (2003) The proteome of Saccharomyces cerevisiae mitochondria. Proc. Natl. Acad. Sci. U. S. A. 100, 13,207–13,212.
Prokisch, H., Scharfe, C., Camp, D. G., 2nd, et al. (2004) Integrative analysis of the mitochondrial proteome in yeast. PLoS Biol. 2, e160.
Reichert, A. S. and Neupert, W. (2004) Mitochondriomics or what makes us breathe. Trends Genet. 20, 555–562.
Scheffler, I. E. (2000) A century of mitochondrial research: achievements and perspectives. Mitochondrion 1, 3–31.
Mortimer, R. K. and Johnston, J. R. (1986) Genealogy of principal strains of the yeast genetic stock center. Genetics 113, 35–43.
Brachmann, C. B., Davies, A., Cost, G. J., et al. D. (1998) Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications. Yeast 14, 115–132.
Gaisne, M., Becam, A. M., Verdiere, J., and Herbert, C. J. (1999) A “natural” mutation in Saccharomyces cerevisiae strains derived from S288c affects the complex regulatory gene HAP1 (CYP1). Curr. Genet. 36, 195–200.
Veal, E. A., Ross, S. J., Malakasi, P., Peacock, E., and Morgan, B. A. (2003) Ybp1 is required for the hydrogen peroxide-induced oxidation of the Yap1 transcription factor. J. Biol. Chem. 278, 30896–30904.
Sherman, F. (1963) Respiration-deficient mutants of yeast. I. Genetics. Genetics 48, 375–385.
Sikorski, R.S. and Hieter, P. (1989) A system of shuttle vectors and host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122, 19–27.
Daum, G., Böhni, P. C., and Schatz, G. (1982) Import of proteins into mitochondria: cytochrome b2 and cytochrome c peroxidase are located in the intermembrane space of yeast mitochondria. J. Biol. Chem. 257, 13,028–13,033.
Diekert, K., de Kroon, A. I. P. M., Kispal, G., and Lill, R. (2001) Isolation and subfractionation of mitochondria from the yeast Saccharomyces cerevisiae. Methods Cell Biol. 65, 37–51.
Glick, B.S. and Pon, L. A. (1995) Isolation of highly purified mitochondria from Saccharomyces cerevisiae. Methods Enzymol. 260, 213–223.
Rowley, N., Prip-Buus, C., Westermann, B., et al. (1994) Mdj1p, a novel chaperone of the DnaJ family, is involved in mitochondrial biogenesis and protein folding. Cell 77, 249–259.
Westermann, B. and Neupert, W. (2000) Mitochondria-targeted green fluorescent proteins: convenient tools for the study of organelle biogenesis in Saccharomyces cerevisiae. Yeast 16, 1421–1427.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2007 Humana Press Inc., Totowa, NJ
About this protocol
Cite this protocol
Altmann, K., Dürr, M., Westermann, B. (2007). Saccharomyces cerevisiae as a Model Organism to Study Mitochondrial Biology. In: Leister, D., Herrmann, J.M. (eds) Mitochondria. Methods in Molecular Biology™, vol 372. Humana Press. https://doi.org/10.1007/978-1-59745-365-3_6
Download citation
DOI: https://doi.org/10.1007/978-1-59745-365-3_6
Publisher Name: Humana Press
Print ISBN: 978-1-58829-667-2
Online ISBN: 978-1-59745-365-3
eBook Packages: Springer Protocols