Abstract
Significant efforts have sought to uncover the protein profile of Arabidopsis mitochondria to act as a model for the mitochondrial proteome from plants. A combination of techniques have been undertaken to achieve this goal. We outline a basic two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) separation of mitochondrial proteins, in-gel trypsination techniques, complex protein lysate digestions, and the identification of proteins by matrix-assisted laser desorption/ionization (MALDI) and electrospray ionization (ESI) mass spectrometry.
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© 2007 Humana Press Inc., Totowa, NJ
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Heazlewood, J.L., Harvey, M.A. (2007). Arabidopsis Mitochondrial Proteomics. In: Leister, D., Herrmann, J.M. (eds) Mitochondria. Methods in Molecular Biology™, vol 372. Humana Press. https://doi.org/10.1007/978-1-59745-365-3_38
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DOI: https://doi.org/10.1007/978-1-59745-365-3_38
Publisher Name: Humana Press
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