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Fluorescence Imaging of Mitochondria in Yeast

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Mitochondria

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 372))

Abstract

The budding yeast Saccharomyces cerevisiae has many advantages as a model system, but until recently high-resolution microscopy was not often attempted in this organism. Its small size, rounded shape, and rigid cell wall were obstacles to exploring the cell biology of this model eukaryote. However, it is now feasible for laboratories to acquire and analyze high-resolution, multidimensional images of yeast cell biology, including the mitochondria. As a result, imaging of yeast has emerged as an important tool in eukaryotic cell biology. This chapter describes labeling methods and optical approaches for visualizing yeast mitochondria using fluorescence microscopy.

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Author information

Authors and Affiliations

  1. Department of Anatomy and Cell Biology, College of Physicians and Surgeons, Columbia University, New York, NY

    Theresa C. Swayne, Anna C. Gay & Liza A. Pon

Authors
  1. Theresa C. Swayne
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  2. Anna C. Gay
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  3. Liza A. Pon
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Editor information

Editors and Affiliations

  1. Department für Biologie I, Botanik, Ludwig-Maximilians-Universität München, München, Germany

    Dario Leister

  2. Zellbiologie, Universität Kaiserslautern, Kaiserslautern, Germany

    Johannes M. Herrmann

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© 2007 Humana Press Inc., Totowa, NJ

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Swayne, T.C., Gay, A.C., Pon, L.A. (2007). Fluorescence Imaging of Mitochondria in Yeast. In: Leister, D., Herrmann, J.M. (eds) Mitochondria. Methods in Molecular Biology™, vol 372. Humana Press. https://doi.org/10.1007/978-1-59745-365-3_31

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  • DOI: https://doi.org/10.1007/978-1-59745-365-3_31

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-667-2

  • Online ISBN: 978-1-59745-365-3

  • eBook Packages: Springer Protocols

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