Abstract
Iodine-125 radioprobing is based on incorporation of radioiodine into a defined position in a nucleic acid molecule. Decay of 125I results in the emission of multiple, low-energy Auger electrons that, along with positively charged residual daughter nuclide, produce DNA strand breaks. The probability of such strand breaks at a given nucleotide is in inverse proportion to the distance from the 125I atom to the sugar of that nucleotide. Therefore, conclusions can be drawn about the conformation or folding of a DNA or RNA molecule based on the distribution of 125I decay-induced strand breaks. Here we describe in detail the application 125I radioprobing for studying the conformation of quadruplex structures, and discuss the advantages and limitations of the method.
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Acknowledgment
This research was supported by the Intramural Research Program of the NIH, Clinical Center.
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Gaynutdinov, T.I., Neumann, R.D., Panyutin, I.G. (2010). Assessing DNA Structures with 125I Radioprobing. In: Baumann, P. (eds) G-Quadruplex DNA. Methods in Molecular Biology, vol 608. Humana Press. https://doi.org/10.1007/978-1-59745-363-9_9
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DOI: https://doi.org/10.1007/978-1-59745-363-9_9
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