Abstract
Kidney tissue laser capture microdissection (LCM) is of great clinical relevance since genome wide studies on total kidney messenger RNA (mRNA) potentially miss important factors involved in the pathogenesis of the disease in glomeruli and tubules. This technique is readily applicable to study mRNA from isolated glomeruli and tubules of human kidney biopsy material. In this chapter we present a “cook-book” practical approach of utilizing LCM in combination with RNA isolation technique in downstream applications in nephrology.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Henger Kretzler M, Doran P, Gene expression fingerprints in human tubulointerstitial inflammation and fibrosis as prognostic markers of disease progression. Kidney Int. 65:904–917,
Higgins JP, Wang L, Kambham N, Montgomery K, Mason V, Vogelmann SU, Lemley KV, Brown PO, Brooks JD, van de Rijn M. Gene expression in the normal adult human kidney assessed by complementary DNA microarray. Mol Biol Cell. 15(2):649–56,
Kretzler M, Cohen CD, Doran P, Repuncturing the renal biopsy: strategies for molecular diagnosis in nephrology. J Am Soc Nephrol. 13:1961–1972,
Henger A, Schmid H, Kretzler M. Gene expression analysis of human renal biopsies: recent developments towards molecular diagnosis of kidney disease. Curr Opin Nephrol Hypertens. 13(3):313–318,
Schwab K, Witte DP, Aronow BJ, Devarajan P, Potter SS, Patterson LT. Microarray analysis of focal segmental glomerulosclerosis. Am J Nephrol. 24(4):438–447,
Devarajan P, Mishra J, Supavekin S, Patterson LT, Potter SS. Gene expression in early ischemic renal injury: clues towards pathogenesis, biomarker discovery, and novel therapeutics. Mol Genet Metab. 80(4):365–376,
Patterson LT, Potter SS. Profiling gene expression in kidney development. Nephron Exp Nephrol. 98(4):e109–113,
Peterson KS, Huang JF, Zhu J, D’Agati V, Liu X, Miller N, Erlander MG, Jackson MR, Winchester RJ. Characterization of heterogeneity in the molecular pathogenesis of lupus nephritis from transcriptional profiles of laser-captured glomeruli. J Clin Invest. 113(12):1722–1733,
Bonner RF, Emmert-Buck M, Cole K, Pohida T, Chuaqui R, Goldstein S, Liotta LA. Laser capture microdissection: Molecular analysis of tissue. Science. 278:1481–1483,
Fend F, Emmert-Buck MR, Chuaqui R, Cole K, Lee J, Liotta LA, Raffeld M. Immuno LCM: Laser capture microdissection of immunostained frozen sections for mRNA analysis. Am J Pathol. 154:61–66,
Kohda Y, Murakami H, Moe OW, Star RA. Analysis of segmental renal gene expression by laser capture microdissection. Kidney Int. 57:321–331,
Woroniecki RP, Schiffer M, Shaw AS, Kaskel FJ, Bottinger EP. Glomerular expression of transforming growth factor-beta (TGF-beta) isoforms in mice lacking CD2-associated protein. Pediatr Nephrol. 21(3):333–338,
Kalluri R, Neilson EG. Epithelial-mesenchymal transition and its implications for fibrosis. J Clin Invest. 112(12):1776–1784,
Tanji N, Ross MD, Cara A, Markowitz GS, Klotman PE, D’Agati VD. Effect of tissue processing on the ability to recover nucleic acid from specific renal tissue compartments by laser capture microdissection. Exp Nephrol. 9(3):229–234, 2001.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2009 Humana Press, a part of Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Woroniecki, R.P., Bottinger, E.P. (2009). Laser Capture Microdissection of Kidney Tissue. In: Becker, G., Hewitson, T. (eds) Kidney Research. Methods in Molecular Biology, vol 466. Humana Press. https://doi.org/10.1007/978-1-59745-352-3_6
Download citation
DOI: https://doi.org/10.1007/978-1-59745-352-3_6
Published:
Publisher Name: Humana Press
Print ISBN: 978-1-58829-945-1
Online ISBN: 978-1-59745-352-3
eBook Packages: Springer Protocols