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Immunocytochemical Methods and Protocols pp 319–326Cite as

Overview of Flow Cytometry and Fluorescent Probes for Flow Cytometry

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 588))

Abstract

This chapter provides an introduction to the use of fluorescent probes in flow cytometry. Sample preparation for the use of surface labeling with antibodies as well as for the use of nucleic acid probes is discussed. The utility of cell sorting is also discussed.

The opinions or assertions herein represent the personal view of the author and are not to be construed as official or as representing the views of the Department of the Army or the Department of Defense

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Author information

Authors and Affiliations

  1. Department of Biophysics, Armed Forces Institute of Pathology, Washington, DC, USA

    Robert E. Cunningham

Authors
  1. Robert E. Cunningham
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Correspondence to Robert E. Cunningham .

Editor information

Editors and Affiliations

  1. Fac. Medicina de Ribeirão Preto, Universidade de São Paulo, Av. Bandeirantes 3900, Ribeirão Preto, 14049-900, Brazil

    Constance Oliver

  2. Fac. Medicina de Ribeirão Preto, Universidade de São Paulo, Av. Bandeirantes 3900, Ribeirão Preto, 14049-900, Brazil

    Maria Célia Jamur

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Cunningham, R.E. (2010). Overview of Flow Cytometry and Fluorescent Probes for Flow Cytometry. In: Oliver, C., Jamur, M. (eds) Immunocytochemical Methods and Protocols. Methods in Molecular Biology, vol 588. Humana Press. https://doi.org/10.1007/978-1-59745-324-0_31

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  • DOI: https://doi.org/10.1007/978-1-59745-324-0_31

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-463-0

  • Online ISBN: 978-1-59745-324-0

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