Abstract
Affinity mass spectrometry is a proteomics approach for selectively isolating target proteins from complex biological fluids for mass spectrometric analysis. When executed in high throughput mode through affinity pipets, the resulting volumetric mass spectrometry arrays enable rapid protein assaying from hundreds of samples. Furthermore, in combination with postcapture proteolytic degradation, this top-down proteomics approach can reveal structural features (i.e., modifications) in the protein sequences that are result of posttranslational modifications and/or point mutations. Described here in greater detail are the individual steps of the high throughput combination of affinity protein capture in antibody-derivatized affinity pipets, protein elution, and protein processing through enzyme-derivatized mass spectrometry targets.
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© 2007 Humana Press Inc., Totowa, NJ
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Nedelkov, D., Kiernan, U.A., Niederkofler, E.E., Tubbs, K.A., Nelson, R.W. (2007). Volumetric Mass Spectrometry Protein Arrays. In: Rampal, J.B. (eds) Microarrays. Methods in Molecular Biology, vol 382. Humana Press. https://doi.org/10.1007/978-1-59745-304-2_21
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DOI: https://doi.org/10.1007/978-1-59745-304-2_21
Publisher Name: Humana Press
Print ISBN: 978-1-58829-944-4
Online ISBN: 978-1-59745-304-2
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