Abstract
A method of high resolution, array-based comparative genomic hybridization is described for the mapping of copy-number changes associated with chromosomal amplifications, deletions, and translocations. The method involves the design of whole-genome or targeted, fine-tiling arrays for synthesis on a high-density digital microarray-synthesis platform. The arrays can span entire eukaryotic genomes or be targeted to specific chromosomal regions for high-resolution identification of copy-number changes and the corresponding breakpoint locations. The methods described include the bioinformatics required for array design, and the protocols for DNA fragmentation, dual-color labeling, microarray hybridization, and array scanning. The processes for data extraction, normalization, and segmentation analysis are also described.
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© 2007 Humana Press Inc., Totowa, NJ
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McCormick, M.R., Selzer, R.R., Richmond, T.A. (2007). Methods in High-Resolution, Array-Based Comparative Genomic Hybridization. In: Rampal, J.B. (eds) Microarrays. Methods in Molecular Biology™, vol 381. Humana Press. https://doi.org/10.1007/978-1-59745-303-5_9
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DOI: https://doi.org/10.1007/978-1-59745-303-5_9
Publisher Name: Humana Press
Print ISBN: 978-1-58829-589-7
Online ISBN: 978-1-59745-303-5
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