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Microarrays pp 121-131 | Cite as

Evaluating the Quality of Data From Microarray Measurements

  • Lili. Wang
  • A. K. Gaigalas
  • M. B. Satterfield
  • M. Salit
  • Y. Zong
  • J. Noble
Protocol
Part of the Methods in Molecular Biology™ book series (MIMB, volume 381)

Abstract

Gene expression technology offers great potentials to generate new insights into human disease pathogenesis; however, the data quality remains a major obstacle for realizing its potentials. In the present study 60-mers oligonucleotide target immobilized on coated glass slides were utilized as a model system to investigate parameters, such as target concentration, retention, signal linearity, and fluorescence properties of fluorophores, which likely affect the quality of microarray results. An array calibration slide was used to calibrate an Axon GenePix 4000A scanner and ensure the dynamic range of the instrument. The work is a first step toward our goal of quantitative gene expression measurements.

Key Words

Cy3 Cy5 oligonucleotide microarray retention scanner calibration signal linearity spectral properties target concentration 

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Copyright information

© Humana Press Inc., Totowa, NJ 2007

Authors and Affiliations

  • Lili. Wang
    • 1
  • A. K. Gaigalas
    • 1
  • M. B. Satterfield
    • 1
  • M. Salit
    • 1
  • Y. Zong
    • 2
  • J. Noble
    • 3
  1. 1.Biotechnology DivisionNational Institute of Standards and TechnologyGaithersburg
  2. 2.Full Moon BioSystems, Inc.Sunnyvale
  3. 3.Biotechnology Group, Quality of Life DivisionNational Physical LaboratoryMiddlesexUK

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