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Microarrays pp 105-119 | Cite as

Detection of DNA Copy Number Alterations in Complex Genomes Using Array Comparative Genomic Hybridization

  • Wei-Wen Cai
Part of the Methods in Molecular Biology™ book series (MIMB, volume 381)

Abstract

Array-based comparative genomic hybridization (array CGH) is becoming a prominent genomic technology with many important applications in biomedical research. Although several platforms of this technology have been published, successful implementation of this technology still demands technical expertise. Here, we describe the technology that has been developed and improved in the past few years are described. Our array CGH technology is primarily based on robust and readily implemented array production method. We also developed related protocols for using our bacterial artifical chromosomes CGH microarrays. This technology was successfully used to detect DNA copy-number alterations in various mouse and human samples.

Key Words

Bacterial artificial chromosomes comparative genomic hybridization DNA copy-number alteration DNA microarray DNA silanization 

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Copyright information

© Humana Press Inc., Totowa, NJ 2007

Authors and Affiliations

  • Wei-Wen Cai
    • 1
  1. 1.Department of Molecular and Human GeneticsHouston

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