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Microarrays pp 401-409 | Cite as

Measurement of the Sugar-Binding Specificity of Lectins Using Multiplexed Bead-Based Suspension Arrays

  • Kazuo Yamamoto
  • Fumiko Yasukawa
  • Seiichiro Ito
Part of the Methods in Molecular Biology™ book series (MIMB, volume 381)

Abstract

A multiplexed glyco-bead array method for determining the sugar-binding specificities of plant lectins was described by a bead-based flow cytometric assay. Glycopeptides with N- and O-glycans were immobilized on multiplexed beads, and the specificities of several kinds of sugar chains were measured in a single reaction. This strategy is easy, rapid, reproducible, and suitable for small samples, and allows the reliable elucidation of sugar-binding properties of lectins under identical conditions.

Key Words

Flow cytometry fluorescent microsphere lectin multiplexed analysis sugar specificity glycopeptide 

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Copyright information

© Humana Press Inc., Totowa, NJ 2007

Authors and Affiliations

  • Kazuo Yamamoto
    • 1
  • Fumiko Yasukawa
    • 2
  • Seiichiro Ito
    • 2
  1. 1.Frontier Science BioscienceUniversity of TokyoKashiwa ChibaJapan
  2. 2.Life Science DivisionHitachi Software Engineering Co. Ltd.KanagawaJapan

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