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Microarrays pp 375-384 | Cite as

Cell Microarray for Functional Exploration of Genomes

  • David Castel
  • Marie-Anne Debily
  • Amandine Pitaval
  • Xavier Gidrol
Part of the Methods in Molecular Biology™ book series (MIMB, volume 381)

Abstract

As more genomes are sequenced, challenge of rapidly unraveling the functions of genes was faced. To that end, cell microarrays have been recently described that permit transfection of thousands of nucleic acids in parallel and enable the analysis of phenotypic consequences of such perturbations. As many parameters can influence the efficiency of transfection and consequently protein expression or extinction, some important features in manufacturing cell microarrays for functional exploration of genomes were described.

Key Words

Cell microarray functional genomics high-throughput screening reverse transfection RNA interference siRNA microarray 

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Copyright information

© Humana Press Inc., Totowa, NJ 2007

Authors and Affiliations

  • David Castel
    • 1
  • Marie-Anne Debily
    • 1
  • Amandine Pitaval
    • 1
  • Xavier Gidrol
    • 1
  1. 1.Functional Genomics DepartmentCEA, DSV, DRREvryFrance

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