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Microarrays pp 279-298 | Cite as

Hybridization Analysis Using Oligonucleotide Probe Arrays

  • Robert S. Matson
  • Jang B. Rampal
Protocol
Part of the Methods in Molecular Biology™ book series (MIMB, volume 381)

Abstract

This chapter describes methodology for the labeling, hybridization, and detection of amplicon target DNA to arrays of oligonucleotide probes attached to plastic substrates. A systematic approach to target discrimination based on both hybridization and wash stringency is provided.

Key Words

Array biotin chemiluminescent discrimination electrophoresis ELF fluorescence hybridization oligonucleotide PCR stringency SYBR green 1 

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Copyright information

© Humana Press Inc., Totowa, NJ 2007

Authors and Affiliations

  • Robert S. Matson
    • 1
  • Jang B. Rampal
    • 2
  1. 1.Beckman Coulter, Inc.Fullerton
  2. 2.Beckman Coulter, Inc.Brea

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