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Poststaining Grids for Transmission Electron Microscopy

Conventional and Alternative Protocols

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Electron Microscopy

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 369))

Abstract

Poststaining ultrathin sections on grids is an essential process in preparing specimens for examination using transmission electron microscopy. This process commonly consists of staining in aqueous solutions of uranyl acetate followed by lead citrate and can result in good consistent quality staining if certain precautions are taken. This chapter covers conventional and alternative methods for poststaining ultrathin sections, including micro-wave-assisted procedures and corrective measures when problems are encountered.

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Author information

Authors and Affiliations

  1. Microscopy and Imaging Center, Texas A & M University, College Station, TX

    E. Ann Ellis

Authors
  1. E. Ann Ellis
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Editor information

Editors and Affiliations

  1. Centre for Microscopy and Microanalysis, The University of Western Australia, Crawley, Western Australia, Australia

    John Kuo

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© 2007 Humana Press Inc.

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Ellis, E.A. (2007). Poststaining Grids for Transmission Electron Microscopy. In: Kuo, J. (eds) Electron Microscopy. Methods in Molecular Biology™, vol 369. Humana Press. https://doi.org/10.1007/978-1-59745-294-6_6

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  • DOI: https://doi.org/10.1007/978-1-59745-294-6_6

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-573-6

  • Online ISBN: 978-1-59745-294-6

  • eBook Packages: Springer Protocols

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