Abstract
Within the last few years real-time quantitative PCR has become the method of choice for the accurate quantification of mRNAlevels. Compared to previous methods the sensitivity of real-time quantitative PCR improved to the detection limit of up to one single molecule per reaction tube. However, the improved sensitivity leads also to higher demands regarding experimental design. Here we describe an approved protocol to establish mRNA quantification by real-time RT qPCR in a straightforward manner.
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC
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Klatte, M., Bauer, P. (2009). Accurate Real-time Reverse Transcription Quantitative PCR. In: Pfannschmidt, T. (eds) Plant Signal Transduction. Methods in Molecular Biology, vol 479. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59745-289-2_4
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DOI: https://doi.org/10.1007/978-1-59745-289-2_4
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-58829-943-7
Online ISBN: 978-1-59745-289-2
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