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Organelle Proteomics

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Two-Dimensional Electrophoresis Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 519))

Summary

The proteome of the cell is at the frontier of being too complex for proteomic analysis. Organelles provide a step up. Organelles compartmentalize the cell enabling a proteome, physiology and metabolism analysis in time and in space. Protein complexes separated by electrophoresis have been identified as the next natural level to characterize the organelles’ compartmentalized membrane and soluble proteomes by mass spectrometry. Work on mitochondria and chloroplasts has shown where we are in the characterization of complex proteomes to understand the network of endogenous and extrinsic factors which regulate growth and development, adaptation and evolution.

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Authors and Affiliations

  1. Department Biology I, University Munich, LMU, Menzingerstr. 67, 80638, Munich

    Matthias Plöscher, Bernhard Granvogl, Veronika Reisinger, Axel Masanek & Lutz Andreas Eichacker

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  1. Matthias Plöscher
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  2. Bernhard Granvogl
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    © 2009 Humana Press, a part of Springer Science+Business Media, LLC

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    Plöscher, M., Granvogl, B., Reisinger, V., Masanek, A., Eichacker, L. (2009). Organelle Proteomics. In: Tyther, R., Sheehan, D. (eds) Two-Dimensional Electrophoresis Protocols. Methods in Molecular Biology, vol 519. Humana Press. https://doi.org/10.1007/978-1-59745-281-6_5

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