Skip to main content

Analysis of Proteins from Marine Molluscs

  • Protocol
  • First Online:
Two-Dimensional Electrophoresis Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 519))

Summary

Application of the two-dimensional gel electrophoresis (2DE) protocols which were developed for samples of mammalian origin gives unsatisfactory results when used in samples from marine molluscs. This chapter describes a detailed protocol of 2DE that can be applied to these organisms, especially for Ruditapes decussatus and Bathymodiolus azoricus.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 169.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Edmond, J.M., Measures, C., Magum, B.M., Grant, B., Sclater, F.R., et al. (1979) On the formation of metal-rich deposits at ridge crests. Earth and Planetary Science Letter 46, 19–30

    Article  CAS  Google Scholar 

  2. Blum, J., and Fridovich, I. (1984) Enzymatic defenses against oxygen toxicity in the hydrothermal vent animals Riftia pachyptila and Calyptogena magnifica. Archives of Biochemistry and Biophysics 228, 617–620

    Article  PubMed  CAS  Google Scholar 

  3. Desbruyères, D., Biscoito, M., Caprais, J.C., Colaço, A., Comtet, T., Crassous, P., et al. (2001) Variations in deep-sea hydrothermal vent communities on the mid-atlantic ridge near the Azores plateau. Deep Sea Research Part I: Oceanographic Research Paper 48, 1325–1346

    Article  Google Scholar 

  4. Géret, F., Rainglet, F., and Cosson, R.P. (1998) Comparison between isolation protocols commonly used for the purification of mollusc metallothioneins. Marine Environmental Research 46, 545–550

    Article  Google Scholar 

  5. Bebianno, M.J., Company, R., Serafim, A., Camus, L., Cosson, R., and Fiala-Médoni, A. (2005) Antioxidant systems and lipid peroxidation in Bathymodiolus Azoricus from mid Atlantic ridge hydrothermal vent fields. Aquatic Toxicology 75, 354–373

    Article  PubMed  CAS  Google Scholar 

  6. Starita-Geribaldi, M., Roux, F., Garin, J., Chevallier, D., Fénichel, P., and Pointis, G., (2003) Development of narrow immobilized pH gradients covering one pH unit for human seminal plasma proteomic analysis. Proteomics 3, 1611–1619

    Article  PubMed  CAS  Google Scholar 

  7. Coelho, M. R., Bebianno, M.J., and Langston, W.J. (2002). Routes of TBT uptake in the clam Ruditapes decussates – Food as vector of TBT uptake. Marine Environmental Research 54, 193–207

    Article  PubMed  CAS  Google Scholar 

  8. Bjellqvist, B., Laird, N., McDowell, M., Olsson, I., and Westermeier, R. (1998). 2–D Electrophoresis, Principles and Methods. Amersham-Biosciences 100 pp

    Google Scholar 

  9. Bradford, M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72, 248–254

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Maria Bebianno .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2009 Humana Press, a part of Springer Science+Business Media, LLC

About this protocol

Cite this protocol

Chora, S., Bebianno, M., Roméo, M. (2009). Analysis of Proteins from Marine Molluscs. In: Tyther, R., Sheehan, D. (eds) Two-Dimensional Electrophoresis Protocols. Methods in Molecular Biology, vol 519. Humana Press. https://doi.org/10.1007/978-1-59745-281-6_12

Download citation

  • DOI: https://doi.org/10.1007/978-1-59745-281-6_12

  • Published:

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-937-6

  • Online ISBN: 978-1-59745-281-6

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics