Summary
Application of the two-dimensional gel electrophoresis (2DE) protocols which were developed for samples of mammalian origin gives unsatisfactory results when used in samples from marine molluscs. This chapter describes a detailed protocol of 2DE that can be applied to these organisms, especially for Ruditapes decussatus and Bathymodiolus azoricus.
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References
Edmond, J.M., Measures, C., Magum, B.M., Grant, B., Sclater, F.R., et al. (1979) On the formation of metal-rich deposits at ridge crests. Earth and Planetary Science Letter 46, 19–30
Blum, J., and Fridovich, I. (1984) Enzymatic defenses against oxygen toxicity in the hydrothermal vent animals Riftia pachyptila and Calyptogena magnifica. Archives of Biochemistry and Biophysics 228, 617–620
Desbruyères, D., Biscoito, M., Caprais, J.C., Colaço, A., Comtet, T., Crassous, P., et al. (2001) Variations in deep-sea hydrothermal vent communities on the mid-atlantic ridge near the Azores plateau. Deep Sea Research Part I: Oceanographic Research Paper 48, 1325–1346
Géret, F., Rainglet, F., and Cosson, R.P. (1998) Comparison between isolation protocols commonly used for the purification of mollusc metallothioneins. Marine Environmental Research 46, 545–550
Bebianno, M.J., Company, R., Serafim, A., Camus, L., Cosson, R., and Fiala-Médoni, A. (2005) Antioxidant systems and lipid peroxidation in Bathymodiolus Azoricus from mid Atlantic ridge hydrothermal vent fields. Aquatic Toxicology 75, 354–373
Starita-Geribaldi, M., Roux, F., Garin, J., Chevallier, D., Fénichel, P., and Pointis, G., (2003) Development of narrow immobilized pH gradients covering one pH unit for human seminal plasma proteomic analysis. Proteomics 3, 1611–1619
Coelho, M. R., Bebianno, M.J., and Langston, W.J. (2002). Routes of TBT uptake in the clam Ruditapes decussates – Food as vector of TBT uptake. Marine Environmental Research 54, 193–207
Bjellqvist, B., Laird, N., McDowell, M., Olsson, I., and Westermeier, R. (1998). 2–D Electrophoresis, Principles and Methods. Amersham-Biosciences 100 pp
Bradford, M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72, 248–254
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Chora, S., Bebianno, M., Roméo, M. (2009). Analysis of Proteins from Marine Molluscs. In: Tyther, R., Sheehan, D. (eds) Two-Dimensional Electrophoresis Protocols. Methods in Molecular Biology, vol 519. Humana Press. https://doi.org/10.1007/978-1-59745-281-6_12
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DOI: https://doi.org/10.1007/978-1-59745-281-6_12
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