Abstract
Co-immunoprecipitation is a common method used to determine protein—protein interactions. This method typically exploits the highly specific interaction between a monoclonal antibody and a protein of interest. Providing that the antibody—protein interaction does not interfere with the ability of the protein to interact with other proteins in a cell lysate, a protein complex containing the antibody, the protein of interest, and its interacting proteins could be isolated on protein A Sepharose beads and purified from the cell lysate. This chapter details many of the techniques and associated procedures of co-immunoprecipitation and highlights many of the concerns and considerations associated with each step of this method.
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© 2007 Humana Press Inc, Totowa, NJ
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Yaciuk, P. (2007). Co-Immunoprecipitation of Protein Complexes. In: Wold, W.S.M., Tollefson, A.E. (eds) Adenovirus Methods and Protocols. Methods in Molecular Medicine™, vol 131. Humana Press. https://doi.org/10.1007/978-1-59745-277-9_8
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DOI: https://doi.org/10.1007/978-1-59745-277-9_8
Publisher Name: Humana Press
Print ISBN: 978-1-58829-901-7
Online ISBN: 978-1-59745-277-9
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