Abstract
Here we describe a collection of methods that have been adapted to produce highly efficient nuclear and cytoplasmic extracts from adenovirus-infected HeLa cells. We describe how to produce extracts from virus-infected cells and how to analyze RNA splicing in vitro using T7 RNA polymerase-derived splicing substrate RNAs.
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© 2007 Humana Press Inc, Totowa, NJ
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Mühlemann], O., Akusjärvi, G. (2007). Preparation of Soluble Extracts From Adenovirus-Infected Cells for Studies of RNA Splicing. In: Wold, W.S.M., Tollefson, A.E. (eds) Adenovirus Methods and Protocols. Methods in Molecular Medicine™, vol 131. Humana Press. https://doi.org/10.1007/978-1-59745-277-9_3
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DOI: https://doi.org/10.1007/978-1-59745-277-9_3
Publisher Name: Humana Press
Print ISBN: 978-1-58829-901-7
Online ISBN: 978-1-59745-277-9
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