Summary
The internalization of activated receptor tyrosine kinases (RTKs) by endocytosis and their subsequent down regulation in lysosomes plays a critical role in regulating the duration and intensity of downstream signaling events. Uncoupling of the RTK cMet from ligand-induced degradation was recently shown to correlate with sustained receptor signaling and increased cell tumorigenicity, suggesting that the corruption of these endocytic mechanisms could contribute to increased cMet signaling in metastatic cancers. To understand how cMet signaling for normal cell growth is controlled by endocytosis and how these mechanisms are dysregulated in metastatic cancers, we developed flow cytometry-based assays to examine cMet internalization.
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Acknowledgments
This work was supported by grants from the National Science Foundation (IBN-343739) and the National Institutes of Health (CA-112605 and CA-119075) to L.A.E. We would like to thank Mark Griffin in the UTMB at Galveston Flow Cytometry and Cell Sorting Core Facility and Marta Lorinczi for their technical assistance.
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Li, N., Hill, K.S., Elferink, L.A. (2008). Analysis of Receptor Tyrosine Kinase Internalization Using Flow Cytometry. In: Vancura, A. (eds) Membrane Trafficking. Methods in Molecular Biology, vol 457. Humana Press. https://doi.org/10.1007/978-1-59745-261-8_23
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DOI: https://doi.org/10.1007/978-1-59745-261-8_23
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