Skip to main content

RNA In Situ Hybridizations on Drosophila Whole Mounts

  • Protocol
Circadian Rhythms

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 362))

Abstract

RNA in situ hybridization is a commonly used technique to achieve spatiotemporal detection of transcripts in tissues. This chapter gives an overview of novel techniques using fluorescent dyes, signal amplification methods, and confocal microscopy in regard to chronobiological applications on Drosophila adult brains.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 219.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Tautz, D., and Pfeifle, C. (1989) A non radioactive in situ hybridization method for the localization of specific RNAs in Drosophila embryos reveals a translational control of the segmentation gene hunchback. Chromosoma 98, 81–85.

    Article  CAS  PubMed  Google Scholar 

  2. Lehmann, R., and Tautz, D. (1994) In situ hybridization to RNA. In: Methods in Cell Biology (Goldstein, L. S. B. and Fyrberg, E. A., eds.). San Diego, CA, Academic Press, pp. 575–598.

    Google Scholar 

  3. Hauptmann, G., and Gerster, T. (1994) Two color whole mount in situ hybridization to vertebrates and Drosophila embryos. Trends Genet. 10, 266.

    Article  CAS  PubMed  Google Scholar 

  4. Cohen, B., and. Cohen, S. M. (1992) Double labelling of mRNA and proteins in Drosophila embryos. In: Nonradioactive Labelling and Detection of Biomolecules (Kessler, C., ed.), Springer-Verlag, Berlin/Heidelberg, pp. 382–392.

    Google Scholar 

  5. Pattatucci, A., and Kaufman, T. (1992) Antibody staining of imaginal discs. DIS 71, 147.

    Google Scholar 

  6. Wülbeck, C., and Simpson, P. (2000) Expression of achaete-scute homologues in discrete proneural clusters of the developing notum of the medfly Ceratitis capitata suggests a common origin for the stereotyped bristle pattern of higher Diptera. Development 127, 1411–1420.

    PubMed  Google Scholar 

  7. Wülbeck, C., and Simpson, P. (2002) The expression of pannier and achaetescute homologues in a mosquito suggests an ancient role of pannier as a selector gene in the regulation of the dorsal body pattern. Development 169, 3861–3871.

    Google Scholar 

  8. Hughes, S. C., and Krause, H. M. (1998) Single and double FISH protocols for Drosophila. In: Confocal Microscopy. Methods and Protocols (Paddock, S. W., ed.). Humana, Totowa, NJ, pp. 93–101.

    Google Scholar 

  9. Nagaso, H., Murara, T., Day, N., and Yokoyama, K. K. (2001) Simultaneous detection of RNA and protein by in situ hybridization and immunological staining. J. Histochem. Cytochem. 49, 1177–1182.

    CAS  PubMed  Google Scholar 

  10. O’Neill, J. W., and Bier, E. (1994) Double-label in situ hybridization using biotin and digoxigenin tagged RNA probes. Biotechniques 17, 870–875.

    CAS  Google Scholar 

  11. Wilkie, G. S., and Davies, I. (1998) Visualizing mRNA by in situ hybridization using high resolution and sensitive tyramide signal amplification. Technical Tips Online, T01458.

    Google Scholar 

  12. Denkers, N., Garcia-Villalba, P., Rodesch, C. K., Nielson, K. R., and Mauch, T.J. (2004) FISHing for chick genes: triple-label whole-mount fluorescence in situ hybridization detects simultaneous and overlapping gene expression in avian embryos. Dev. Dyn. 229, 651–657.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2007 Humana Press Inc.

About this protocol

Cite this protocol

Wülbeck, C., Helfrich-Förster, C. (2007). RNA In Situ Hybridizations on Drosophila Whole Mounts. In: Rosato, E. (eds) Circadian Rhythms. Methods in Molecular Biology™, vol 362. Humana Press. https://doi.org/10.1007/978-1-59745-257-1_40

Download citation

  • DOI: https://doi.org/10.1007/978-1-59745-257-1_40

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-417-3

  • Online ISBN: 978-1-59745-257-1

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics