Abstract
In the filamentous fungus Neurospora crassa the production of asexual spores (conidia) is regulated by its circadian clock. When the fungus is grown on a thin layer of agar medium in long growth tubes (so-called “race tubes”), restricting its growth to one direction only, bright orange bands are clearly visible. This banding pattern persists with a periodicity of approx 24 h in the absence of any environmental stimuli. The bands are formed by alternating zones of nonsporulating mycelium and mycelium laden with orange conidia. Assaying Neurospora conidiation on race tubes is a simple yet powerful and versatile tool for studying the circadian clock of this model organism.
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References
Brandt, W. H. (1953) Zonation in a prolineless strain of Neurospora. Mycologia 45, 194–208.
Pittendrigh, C. S., Bruce, V. G., Rosenzweig, N. S., and Rubin, M. L. (1959) A biological clock in Neurospora. Nature 184, 169–170.
Sargent, M. L., Briggs, W. R., and Woodward, D. O. (1966) Circadian nature of a rhythm expressed by an invertaseless strain of Neurospora crassa. Plant Physiol. 41, 1343–1349.
Sargent, M. L., and Briggs, W. R. (1967) The effect of light on a circadian rhythm of conidiation in Neurospora. Plant Physiol. 42, 1504–1510.
Feldman, J. F., and Hoyle, M. N. (1974) A direct comparison between circadian and non-circadian rhythms in Neurospora crassa. Plant Physiol. 53, 928–930.
Francis, C. D., and Sargent, M. L. (1979) Effects of temperature perturbations on circadian conidiation in Neurospora. Plant Physiol. 64, 1000–1004.
Loros, J. J,. and Dunlap, J. C. (2001) Circadian rhythms in Neurospora. Annu. Rev. Physiol. 63, 757–794.
Roenneberg, T., and Merrow, M. (2001) Seasonality and photoperiodism in fungi. J. Biol. Rhythms 16, 403–414.
Springer, M. L. (1993) Genetic control of fungal differentiation: the three sporulation pathways of Neurospora crassa. BioEssays 15, 365–374.
Davis, R. H. (2000) Neurospora: Contributions of a Model Organism. Oxford University Press, New York.
Ryan, F. J., Beadle, G. W., and Tatum, E. L. (1943) The tube method of measuring growth rate of Neurospora. Am. J. Bot. 30, 784–799.
Sargent, M. L,. and Woodward, D. O. (1969) Genetic determinants of circadian rhythmicity in Neurospora. J. Bacteriol. 97, 861–866.
Sargent, M. L., and Kaltenborn, S. H. (1972) Effects of media composition and carbon dioxide on circadian conidiation in Neurospora. Plant Physiol. 50, 171–175.
Roenneberg, T., and Taylor, W. (2000) Automated recordings of bioluminescence with special reference to the analysis of circadian rhythms. Methods Enzymol. 305, 104–119.
Gooch, V., and Thoen, J. Time lapse video of Neurospora circadian conidiation rhythms. http://www.mrs.umn.edu/%7Egoochv/Circadian/circadian.html
Gooch, V. D., Freeman, L., and Lakin-Thomas, P. L. (2004) Time-lapse analysis of the circadian rhythms of conidiation and growth rate in Neurospora. J. Biol. Rhythms 19, 493–503.
Pittendrigh, C. S. (1960) Circadian rhythms and the circadian organization of living things. Cold Spring Harbor Symp. Quant. Biol. 25, 159–184.
Kramer, C., Loros, J. J., Dunlap, J. C., and Crosthwaite, S. K. (2003) Role for antisense RNA in regulating circadian clock function in Neurospora crassa. Nature 421, 948–952.
Johnson, C. H. (1999) Forty years of PRCs: what have we learned. Chronobiol. Int. 16, 711–743.
Merrow, M., Brunner, M., and Roenneberg, T. (1999) Assignment of circadian function for the Neurospora clock gene frequency. Nature 399, 584–586.
Pregueiro, A. M., Price-Lloyd, N., Bell-Pedersen, D., Heintzen, C., Loros J. J., and Dunlap, J. C. (2005) Assignment of an essential role for the Neurospora frequency gene in circadian entrainment to temperature cycles. Proc. Natl. Acad. Sci. USA 102, 2210–2215.
Vogel, H. J. (1956) A convenient growth medium for Neurospora (Medium N). Microbiol. Genet. Bull. 13, 42–43.
Fries, N. (1938) Uber die Bedeutung von Wuchsstoffen für das Wachstum verschiedener Pilze. Symbolae Botan. Upsalienses 3, 1–188.
White, B., and Woodward, D. (1995) A simple method for making disposable race tubes. http://www.fgsc.net/fgn42/white.html
Davis, R.H. and de Serres, F.J. (1970) Genetic and microbial research techniques for Neurospora crassa. Methods Enzymol. 17A, 79–143.
Crosthwaite, S. K., and Kramer, C. (2004) Clock gene antisense RNA and circadian timing in Neurospora crassa. In: Circadian Clock in Eukaryotic Microbes (Kippert, F., ed.) Landes Bioscience, Georgetown, TX, In press, on-line release Dec 2004 (http://www.eurekah.com).
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Kramer, C. (2007). Rhythmic Conidiation in Neurospora crassa . In: Rosato, E. (eds) Circadian Rhythms. Methods in Molecular Biology™, vol 362. Humana Press. https://doi.org/10.1007/978-1-59745-257-1_3
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DOI: https://doi.org/10.1007/978-1-59745-257-1_3
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