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Rhythmic Conidiation in Neurospora crassa

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Circadian Rhythms

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 362))

Abstract

In the filamentous fungus Neurospora crassa the production of asexual spores (conidia) is regulated by its circadian clock. When the fungus is grown on a thin layer of agar medium in long growth tubes (so-called “race tubes”), restricting its growth to one direction only, bright orange bands are clearly visible. This banding pattern persists with a periodicity of approx 24 h in the absence of any environmental stimuli. The bands are formed by alternating zones of nonsporulating mycelium and mycelium laden with orange conidia. Assaying Neurospora conidiation on race tubes is a simple yet powerful and versatile tool for studying the circadian clock of this model organism.

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© 2007 Humana Press Inc.

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Kramer, C. (2007). Rhythmic Conidiation in Neurospora crassa . In: Rosato, E. (eds) Circadian Rhythms. Methods in Molecular Biology™, vol 362. Humana Press. https://doi.org/10.1007/978-1-59745-257-1_3

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  • DOI: https://doi.org/10.1007/978-1-59745-257-1_3

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-417-3

  • Online ISBN: 978-1-59745-257-1

  • eBook Packages: Springer Protocols

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