Abstract
This chapter deals with methods of protein extraction from cyanobacterial cells based on work in the circadian model organism Synechococcus elongatus PCC 7942. Some of these techniques have already been used successfully for analysis of circadian rhythms in cyanobacteria, whereas others are heretofore unpublished, but may yield exciting results in the near future.
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References
Nakahira, Y., Katayama, M., Miyashita, H., et al. (2004) Global gene repression by KaiC as a master process of prokaryotic circadian system. Proc. Natl. Acad. Sci. USA 101, 881–885.
Ouyang, Y., Andersson, C. R., Kondo, T., Golden, S. S., and Johnson, C. H. (1998) Resonating circadian clocks enhance fitness in cyanobacteria. Proc. Natl. Acad. Sci. USA 95, 8660–8664.
Kageyama, H., Kondo, T., and Iwasaki, H. (2003) Circadian formation of clock protein complexes by KaiA, KaiB, KaiC, and SasA in cyanobacteria. J. Biol. Chem. 278, 2388–2395.
Iwasaki, H., Taniguchi, Y., Ishiura, M., and Kondo, T. (1999) Physical interactions among circadian clock proteins KaiA, KaiB and KaiC in cyanobacteria. EMBO J. 18, 1137–1145.
Iwasaki, H., Nishiwaki, T., Kitayama, Y., Nakajima, M., and Kondo, T. (2002) KaiA-stimulated KaiC phosphorylation in circadian timing loops in cyanobacteria. Proc. Natl. Acad. Sci. USA 99, 15,788–15,793.
Nair, U., Ditty, J. L., Min, H., and Golden, S. S. (2002) Roles for sigma factors in global circadian regulation of the cyanobacterial genome. J. Bacteriol. 184, 3530–3538.
Mutsuda, M., Michel, K.-P., Zhang, X., Montgomery, B. L., and Golden, S. S. (2003) Biochemical properties of CikA, an unusual phytochrome-like histidine protein kinase that resets the circadian clock in Synechococcus elongatus PCC 7942. J. Biol. Chem. 278, 19,102–19,110.
Kitayama, Y., Iwasaki, H., Nishiwaki, T., and Kondo, T. (2003) KaiB functions as an attenuator of KaiC phosphorylation in the cyanobacteria circadian clock system. EMBO J. 22, 1–8.
Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual. 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
The QIAexpressionist: A Handbook for High-Level Expression and Purification of 6x His-Tagged Proteins. 5th ed. Qiagen, Valencia, CA.
Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J. (1951) Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265–275.
Fulda, S., Mikkat, S., Schroder, W., and Hagemann, M. (1999) Isolation of saltinduced periplasmic proteins from Synechocystis sp. strain PCC 6803. Arch. Microbiol. 171, 214–217.
Norling, B., Zak, E., Andersson, B., and Pakrasi, H. (1998) 2D-isolation of pure plasma and thylakoid membranes from the cyanobacterium Synechocystis sp. PCC 6803. FEBS Lett. 436, 189–192.
Zak, E., Norling, B., Andersson, B., and Pakrasi, H. B. (1999) Subcellular localization of the BtpA protein in the cyanobacterium Synechocystis sp. PCC 6803. Eur. J. Biochem. 261, 311–316.
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Ivleva, N.B., Golden, S.S. (2007). Protein Extraction, Fractionation, and Purification From Cyanobacteria. In: Rosato, E. (eds) Circadian Rhythms. Methods in Molecular Biology™, vol 362. Humana Press. https://doi.org/10.1007/978-1-59745-257-1_26
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DOI: https://doi.org/10.1007/978-1-59745-257-1_26
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