Live Imaging of Drosophila Myoblast Fusion
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Myoblast fusion requires a number of cellular behaviors, including cell migration, recognition, and adhesion, as well as a series of subcellular behaviors, such as cytoskeletal rearrangements, vesicle trafficking, and membrane dynamics, leading to two cells becoming one. With the discovery of fluorescent proteins that can be introduced and studied within living cells, the possibility of monitoring these complex processes within the living embryo is now a reality. Live imaging, unlike imaging techniques for fixed embryos, allows the opportunity to visualize and measure the dynamics of these processes in vivo. This chapter describes the development and use of live imaging techniques to study myoblast fusion in Drosophila.
Key WordsDrosophila myoblast fusion muscle development fluorescent proteins live imaging
We thank members of Baylies' laboratory, Owen Richardson, and especially Kat Hadjantonakis for stimulating discussions and advice. We also recognize the valuable input from Julia Kaltschmidt during our early days of filming. This work is supported by NIH grants GM56989 and GM78318 to M.B.
- 10.Haseloff, J., Dormand, E., and Brand, A. H. (1998) Methods Mol. Biol. 122, 241–259.Google Scholar
- 25.Barolo, S., Carver, L. A., and Posakony, J. W. (2000) Biotechniques 29, 726, 728, 730, 732.Google Scholar
- 32.Stute, C., Kesper, D., Holz, A., Buttgere, D., and Renkerwitz-Ashl, R. (2006) Establishment of cell type specific Galy-driver lines for the mesoderm of dro-sophila. Dros. Inf. Serv. 89, 111–115.Google Scholar
- 33.Kocherlakota, K. S., Wu, J. M., McDermott, J., and Abmayr, S. M. (2008) Analysis of the Cell Adhesion Molecule Sticks-and-Stones Reveals Multiple Redundant Functional Domains, Protein-Interaction Motifs and Phosphorylated Tyrosines That Direct Myoblast Fusion in Drosophila melanogaster. Genetics 178, 1371–1383.CrossRefPubMedGoogle Scholar