Cell Fusion pp 263-274 | Cite as

Live Imaging of Drosophila Myoblast Fusion

  • Brian E. Richardson
  • Karen Beckett
  • Mary K. Baylies
Part of the Methods in Molecular Biology™ book series (MIMB, volume 475)


Myoblast fusion requires a number of cellular behaviors, including cell migration, recognition, and adhesion, as well as a series of subcellular behaviors, such as cytoskeletal rearrangements, vesicle trafficking, and membrane dynamics, leading to two cells becoming one. With the discovery of fluorescent proteins that can be introduced and studied within living cells, the possibility of monitoring these complex processes within the living embryo is now a reality. Live imaging, unlike imaging techniques for fixed embryos, allows the opportunity to visualize and measure the dynamics of these processes in vivo. This chapter describes the development and use of live imaging techniques to study myoblast fusion in Drosophila.

Key Words

Drosophila myoblast fusion muscle development fluorescent proteins live imaging 



We thank members of Baylies' laboratory, Owen Richardson, and especially Kat Hadjantonakis for stimulating discussions and advice. We also recognize the valuable input from Julia Kaltschmidt during our early days of filming. This work is supported by NIH grants GM56989 and GM78318 to M.B.


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Copyright information

© Humana Press, a part of Springer Science + Business Media, LLC 2008

Authors and Affiliations

  • Brian E. Richardson
    • 1
  • Karen Beckett
    • 1
  • Mary K. Baylies
    • 1
  1. 1.Program in Developmental BiologyMemorial Sloan Kettering Institute and Weill Graduate School at Cornell Medical SchoolNew York

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