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Ultrastructural Imaging of Cell Fusion in Caenorhabditis elegans

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Cell Fusion

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 475))

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Summary

Caenorhabditis elegans is a well-established model system particularly suited for studying cell–cell fusion because of its highly predictable and rapid development and its known cell lineage. This chapter focuses on understanding the ultrastructural components of cell fusion through the use of transmission electron microscopy (TEM). Published TEM studies have described the initial demonstration of syncytial cells in the worm, the vesiculation of the bilayers between cells during widening of the normal fusion aperture, and the appearance of microfusion intermediates in the membranes of cells with fusion-defective mutations. Capturing events observed in embryos on the light microscope and preserving the integrity of cellular membranes for examination by TEM require some special considerations that differ from many ultrastructural studies of cells. The principles of different techniques for TEM and details of protocols that have been used to investigate cell fusion in the nematode are discussed in this chapter.

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Authors and Affiliations

  1. Department of Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, CT

    Star Ems & William A. Mohler

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  1. Star Ems
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  2. William A. Mohler
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Editors and Affiliations

  1. Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD

    Elizabeth H. Chen

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© 2008 Humana Press, a part of Springer Science + Business Media, LLC

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Ems, S., Mohler, W.A. (2008). Ultrastructural Imaging of Cell Fusion in Caenorhabditis elegans . In: Chen, E.H. (eds) Cell Fusion. Methods in Molecular Biology™, vol 475. Humana Press. https://doi.org/10.1007/978-1-59745-250-2_14

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  • DOI: https://doi.org/10.1007/978-1-59745-250-2_14

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-911-6

  • Online ISBN: 978-1-59745-250-2

  • eBook Packages: Springer Protocols

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