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Cell Fusion pp 245-262 | Cite as

Ultrastructural Imaging of Cell Fusion in Caenorhabditis elegans

  • Star Ems
  • William A. Mohler
Protocol
  • 1.8k Downloads
Part of the Methods in Molecular Biology™ book series (MIMB, volume 475)

Summary

Caenorhabditis elegans is a well-established model system particularly suited for studying cell–cell fusion because of its highly predictable and rapid development and its known cell lineage. This chapter focuses on understanding the ultrastructural components of cell fusion through the use of transmission electron microscopy (TEM). Published TEM studies have described the initial demonstration of syncytial cells in the worm, the vesiculation of the bilayers between cells during widening of the normal fusion aperture, and the appearance of microfusion intermediates in the membranes of cells with fusion-defective mutations. Capturing events observed in embryos on the light microscope and preserving the integrity of cellular membranes for examination by TEM require some special considerations that differ from many ultrastructural studies of cells. The principles of different techniques for TEM and details of protocols that have been used to investigate cell fusion in the nematode are discussed in this chapter.

Key Words:

Cell fusion; ultrastructure; transmission electron microscopy; Caenorhabditis elegans

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Copyright information

© Humana Press, a part of Springer Science + Business Media, LLC 2008

Authors and Affiliations

  • Star Ems
    • 1
  • William A. Mohler
    • 1
  1. 1.Department of Genetics and Developmental BiologyUniversity of Connecticut Health CenterFarmington

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