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Cell Fusion pp 165-196 | Cite as

Cell Fusion Assays for Yeast Mating Pairs

  • Eric Grote
Protocol
Part of the Methods in Molecular Biology™ book series (MIMB, volume 475)

Summary

Yeast mating provides an accessible genetic system for the discovery of fundamental mechanisms in eukaryotic cell fusion. Although aspects of yeast mating related to pheromone signaling and polarized growth have been intensively investigated, fusion itself is poorly understood. This chapter describes methods for measuring the overall efficiency of yeast cell fusion and for monitoring various stages of the fusion process including cell wall remodeling, plasma membrane fusion, and nuclear fusion.

Key Words

Cell fusion mating membrane fusion cell wall remodeling karyogamy 

Notes

Acknowledgments

This work was supported by a Research Scholar Award from the American Cancer Society. Scott Nolan participated in the development of the karyogamy, vacuole fusion, and permeance assays.

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Copyright information

© Humana Press, a part of Springer Science + Business Media, LLC 2008

Authors and Affiliations

  • Eric Grote
    • 1
  1. 1.Department of Biochemistry and Molecular BiologyJohns Hopkins Bloomberg School of Public HealthBaltimore

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