Summary
Yeast mating provides an accessible genetic system for the discovery of fundamental mechanisms in eukaryotic cell fusion. Although aspects of yeast mating related to pheromone signaling and polarized growth have been intensively investigated, fusion itself is poorly understood. This chapter describes methods for measuring the overall efficiency of yeast cell fusion and for monitoring various stages of the fusion process including cell wall remodeling, plasma membrane fusion, and nuclear fusion.
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Notes
- 1.
* Use of pEG463 requires a material transfer agreement from R. Tsien, UCSD, for mCherry (21).
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Acknowledgments
This work was supported by a Research Scholar Award from the American Cancer Society. Scott Nolan participated in the development of the karyogamy, vacuole fusion, and permeance assays.
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© 2008 Humana Press, a part of Springer Science + Business Media, LLC
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Grote, E. (2008). Cell Fusion Assays for Yeast Mating Pairs. In: Chen, E.H. (eds) Cell Fusion. Methods in Molecular Biology™, vol 475. Humana Press. https://doi.org/10.1007/978-1-59745-250-2_10
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DOI: https://doi.org/10.1007/978-1-59745-250-2_10
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