Abstract
Transcription-based reporters have been instrumental in characterizing the Wnt/β-catenin signaling pathway and will be essential in the search for therapeutics aimed at combating diseases linked to aberrant signaling. In this chapter, we introduce a new improved Wnt/β-catenin reporter system, β-catenin-activated reporter (BAR), and its accompanying control reporter system, found unresponsive BAR (fuBAR). Its enhanced sensitivity, increased dynamic range, and lentiviral platform provide a reporter system that will keep pace with the needs of scientists in the field.
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We thank the Howard Hughes Medical Institute for funding.
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© 2008 Humana Press, a part of Springer Science+Business Media, LLC
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Biechele, T.L., Moon, R.T. (2008). Assaying β-Catenin/TCF Transcription with β-Catenin/TCF Transcription-Based Reporter Constructs. In: Vincan, E. (eds) Wnt Signaling. Methods in Molecular Biology™, vol 468. Humana Press. https://doi.org/10.1007/978-1-59745-249-6_8
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DOI: https://doi.org/10.1007/978-1-59745-249-6_8
Publisher Name: Humana Press
Print ISBN: 978-1-58829-912-3
Online ISBN: 978-1-59745-249-6
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