Abstract
Many aspects of animal development are dependent on the dynamic release of calcium (Ca2+) ions. Although Ca2+ release within a cell is tightly controlled, how the release dynamics result in a specific biological output in embryonic development is less clear. The integration of pharmacological and molecular-genetic studies with in vivo imaging in zebrafish and Xenopus has linked endogenous Ca2+ release to the Wnt signaling network. Our data suggests that distinct Ca2+ release dynamics lead to antagonism of the developmentally important Wnt/ß-catenin pathway while sustained Ca2+ release modulates polarized cell movements.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Tsien, R. Y., Pozzan, T., and Rink, T. J. (1982) Calcium homeostasis in intact lymphocytes: cytoplasmic free calcium monitored with a new, intracellularly trapped fluorescent indicator. J Cell Biol 94, 325–334.
Thomas, D., Tovey, S. C., Collins, T. J., Bootman, M. D., Berridge, M. J., and Lipp, P. (2000) A comparison of fluorescent Ca2+ indicator properties and their use in measuring elementary and global Ca2+ signals. Cell Calcium 28, 213–223.
Shimomura, O. (2005) The discovery of aequorin and green fluorescent protein. J Microsc 217, 1–15.
Grynkiewicz, G., Poenie, M., and Tsien, R. Y. (1985) A new generation of Ca2+ indicators with greatly improved fluorescence properties. J Biol Chem 260, 3440–3450.
Slusarski, D. C., Corces, V. G., and Moon, R. T. (1997) Interaction of Wnt and a Frizzled homologue triggers G-protein-linked phosphatidylinositol signalling. Nature 390, 410–413.
Slusarski, D. C., Yang-Snyder, J., Busa, W. B., and Moon, R. T. (1997) Modulation of embryonic intracellular Ca2+ signaling by Wnt-5A. Dev Biol 185, 114–120.
Westfall, T. A., Brimeyer, R., Twedt, J., Gladon, J., Olberding, A., Furutani-Seiki, M., and Slusarski, D. C. (2003) Wnt-5/pipetail functions in vertebrate axis formation as a negative regulator of Wnt/{beta}-catenin activity. J Cell Biol 162, 889–898.
Liu, X. X., Liu, T., Slusarski, D. C., Yang-Snyder, J., Malbon, C. C., Moon, R. T., and Wang, H. Y. (1999) Activation of a Frizzled-2/beta-adrenergic receptor chimera promotes Wnt signaling and differentiation of mouse F9 teratocarcinoma cells via G alpha o and G alpha t. Proc Natl Acad Sci USA 96, 14383–14388.
Westerfield, M. (1995) The zebrafish book: A guide for the laboratory use of zebrafish. University of Oregon Press, Eugene.
Nieukoop, P. D., and Faber, J. (1994) Normal table of Xenopus laevis (Daudin): a systematical and chronological survey of the development from the fertilized egg till the end of metamorphosis. Garland Publishing, New York, London.
Westfall, T. A., Hjertos, B., and Slusarski, D. C. (2003) Requirement for intracellular calcium modulation in zebrafish dorsal-ventral patterning. Dev Biol 259, 380–391.
Ahumada, A., Slusarski, D. C., Liu, X. X., Moon, R. T., Malbon, C. C., and Wang, H. Y. (2002) Signaling of rat Frizzled-2 through phosphodiesterase and cyclic GMP. Science 298, 2006–2010.
Acknowledgments
This work was supported by RO1 CA112369 to DCS, the Roy J. Carver Charitable Trust to DWH, and An American Heart AssoCitation predoctoral fellowship to CMF.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2008 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Freisinger, C.M., Houston, D.W., Slusarski, D.C. (2008). Image Analysis of Calcium Release Dynamics. In: Vincan, E. (eds) Wnt Signaling. Methods in Molecular Biology™, vol 468. Humana Press. https://doi.org/10.1007/978-1-59745-249-6_11
Download citation
DOI: https://doi.org/10.1007/978-1-59745-249-6_11
Publisher Name: Humana Press
Print ISBN: 978-1-58829-912-3
Online ISBN: 978-1-59745-249-6
eBook Packages: Springer Protocols