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Generation of Adipose Tissue-Specific Transgenic Mouse Models

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Adipose Tissue Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 456))

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Summary

Adipose tissue plays a critical role in energy homeostasis, not only in storing triglycerides, but also in responding to nutrient, neural, and hormonal signals, and secreting adipokines that control feeding, thermogenesis, immunity, and neuroendocrine function. It is conceivable that adipose tissue-specific gene expression would influence the aforementioned functions. A feasible approach to prepare adipose tissue-specific transgenic mouse models is necessary for such studies. Here, we report the preparation of adipose tissue-specific cholesteryl ester transfer protein transgenic mice. The general principle might apply to the establishment of other adipose tissue-specific transgenic mice models.

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References

  1. Flier JS (2004) Obesity wars: molecular progress confronts an expanding epidemic Cell 116:337–350

    Article  CAS  PubMed  Google Scholar 

  2. Ahima RS (2006) Adipose tissue as an endocrine organ. Obesity Suppl 5:242S–249S

    Article  Google Scholar 

  3. Weisberg SP, McCann D, Desai M, Rosenbaum M, Leibel RL, and Ferrante AW Jr (2003) Obesity is associated with macrophage accumulation in adipose tissue. J Clin Invest 112:1796–1808

    CAS  PubMed  Google Scholar 

  4. Xu H, Barnes GT, Yang Q, et al (2003) Chronic inflammation in fat plays a crucial role in the development of obesity-related insulin resistance. J Clin Invest 112:1821–1830

    CAS  PubMed  Google Scholar 

  5. Skurk T, Hauner H (2004) Obesity and impaired fibrinolysis: role of adipose production of plasminogen activator inhibitor-1. Int J Obes Relat Metab Disord 28:1357–1364

    Article  CAS  PubMed  Google Scholar 

  6. Belanger C, Luu-The V, Dupont P, Tchernof A (2002) Adipose tissue intracrinology: potential importance of local androgen/estrogen metabolism in the regulation of adiposity. Horm Metab Res 34:737–745

    Article  CAS  PubMed  Google Scholar 

  7. Morton NM, Paterson JM, Masuzaki H, et al (2004) Novel adipose tissue-mediated resistance to diet-induced visceral obesity in 11 beta-hydroxysteroid dehydrogenase type 1-deficient mice. Diabetes 53:931–938

    Article  CAS  PubMed  Google Scholar 

  8. Shepherd PR, Gnudi L, Tozzo E, Yang H, Leach F, Kahn BB (1993) Adipose cell hyperplasia and enhanced glucose disposal in transgenic mice overexpressing GLUT4 selectively in adipose tissue. J Biol Chem 268:22243–22246

    CAS  PubMed  Google Scholar 

  9. Kopecky J, Clarke G, Enerback S, Spiegelman B, Kozak LP (1995) Expression of the mitochondrial uncoupling protein gene from the aP2 gene promoter prevents genetic obesity. J Clin Invest 96:2914–2923

    Article  CAS  PubMed  Google Scholar 

  10. Horton JD, Shimomura I, Ikemoto S, Bashmakov Y, Hammer RE (2003) Overexpression of sterol regulatory element-binding protein-1a in mouse adipose tissue produces adipocyte hypertrophy, increased fatty acid secretion, and fatty liver. J Biol Chem 278: 36652–36660

    Article  CAS  PubMed  Google Scholar 

  11. Jurczak MJ, Danos AM, Rehrmann VR, Allison MB, Greenberg CC, Brady MJ (2007) Transgenic overexpression of protein targeting to glycogen markedly increases adipocytic glycogen storage in mice. Am J Physiol Endocrinol Metab 292:E952–E963

    Article  CAS  PubMed  Google Scholar 

  12. Barlow C, Schroeder M, Lekstrom-Himes J, Kylefjord H, Deng CX, Wynshaw-Boris A, Spiegelman BM, Xanthopoulos KG (1997) Targeted expression of Cre recombinase to adipose tissue of transgenic mice directs adipose-specific excision of loxP-flanked gene segments. Nucleic Acids Res 25:2543–2545

    Article  CAS  PubMed  Google Scholar 

  13. Tall AR (1993) Plasma cholesteryl ester transfer protein. J Lipid Res 120:1255–1274

    Google Scholar 

  14. Agellon LB, Walsh A, Hayek T, Moulin P, Jiang XC, Shelanski SA, Breslow JL, Tall AR (1991) Reduced high density lipoprotein cholesterol in human cholesteryl ester transfer protein transgenic mice. J Biol Chem 266:10796–1080

    CAS  PubMed  Google Scholar 

  15. Jiang XC, Moulin P, Quinet E, Goldberg IJ, Yacoub LK, Agellon LB, Compton D, Schnitzer-Polokoff R, Tall AR (1991) Mammalian adipose tissue and muscle are major sources of lipid transfer protein mRNA. J Biol Chem 266:4631–4639

    CAS  PubMed  Google Scholar 

  16. Quinet EM, Huerta P, Nancoo D, Tall AR, Marcel YL, McPherson R (1993) Adipose tissue cholesteryl ester transfer protein mRNA in response to probucol treatment: cholesterol and species dependence. J Lipid Res 34:845–852

    CAS  PubMed  Google Scholar 

  17. Quinet E, Tall A, Ramakrishnan R, Rudel L (1991) Plasma lipid transfer protein as a determinant of the atherogenicity of monkey plasma lipoproteins. J Clin Invest 87:1559–1566

    Article  CAS  PubMed  Google Scholar 

  18. Radeau T, Lau P, Robb M, McDonnell M, Ailhaud G, McPherson R (1995) Cholesteryl ester transfer protein (CETP) mRNA abundance in human adipose tissue: relationship to cell size and membrane cholesterol content. J Lipid Res 36:2552–2561

    CAS  PubMed  Google Scholar 

  19. Dullaart RP, Sluiter WJ, Dikkeschei LD, Hoogenberg K, Van Tol A (1994) Effect of adiposity on plasma lipid transfer protein activities: a possible link between insulin resistance and high density lipoprotein metabolism. Eur J Clin Invest 24:188–194

    Article  CAS  PubMed  Google Scholar 

  20. Arai T, Yamashita S, Hirano K, Sakai N, Kotani K, Fujioka S, Nozaki S, Keno Y, Yamane M, Shinohara E. (1994) Increased plasma cholesteryl ester transfer protein in obese subjects. A possible mechanism for the reduction of serum HDL cholesterol levels in obesity. Arterioscler Thromb 14:1129–1136

    CAS  PubMed  Google Scholar 

  21. Remillard P, Shen G, Milne R, Maheux P (2001) Induction of cholesteryl ester transfer protein in adipose tissue and plasma of the fructose-fed hamster. Life Sci 69:677–687

    Article  CAS  PubMed  Google Scholar 

  22. Shen GX, Angel A (1995) Regulation of cholesteryl ester transfer activity in adipose tissue: comparison between hamster and rat species. Am J Physiol 269:99–107

    Google Scholar 

  23. Zhou H, Li Z, Hojjati MR, Jang D, Beyer TP, Cao G, Tall AR, Jiang XC (2006) Adipose tissue-specific CETP expression in mice: impact on plasma lipoprotein metabolism. J Lipid Res 47:2011–2019

    Article  CAS  PubMed  Google Scholar 

  24. Jiang XC, Agellon LB, Walsh A, Breslow JL, Tall A (1992) Dietary cholesterol increases transcription of the human cholesteryl ester transfer protein gene in transgenic mice. Dependence on natural flanking sequences. J Clin Invest 90:1290–1295

    Article  CAS  PubMed  Google Scholar 

  25. Yvan-Charvet L, Even P, Bloch-Faure M, Guerre-Millo M, Moustaid-Moussa N, Ferre P, Quignard-Boulange A (2005) Deletion of the angiotensin type 2 receptor (AT2R) reduces adipose cell size and protects from diet-induced obesity and insulin resistance. Diabetes 54:991–999

    Article  CAS  PubMed  Google Scholar 

  26. Fu Y, Luo N, Lopes-Virella MF, Garvey WT (2002) The adipocyte lipid binding protein (ALBP/aP2) gene facilitates foam cell formation in human THP-1 macrophages. Atherosclerosis 165:259–269

    Article  CAS  PubMed  Google Scholar 

  27. Chen HC, Farese RV Jr. (2002) Determination of adipocyte size by computer image analysis. J Lipid Res 43:986–989

    CAS  PubMed  Google Scholar 

  28. Le Lay S, Ferre P, Dugail I (2004) Adipocyte cholesterol balance in obesity. Biochem Soc Trans 32:103–106

    Article  PubMed  Google Scholar 

  29. Farley FW, Soriano P, Steffen LS, Dymecki SM (2000) Widespread recombinase expression using FLPeR (flipper) mice. Genesis 28:106–110

    Article  CAS  PubMed  Google Scholar 

  30. Jones JR, Shelton KD, Magnuson MA (2005) Strategies for the use of site-specific recombinases in genome engineering. Methods Mol Med 103:245–257

    CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Anatomy and Cell Biology, SUNY Downstate Medical Center, Brooklyn, New York, USA

    Xian-Cheng Jiang

Authors
  1. Xian-Cheng Jiang
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Editor information

Editors and Affiliations

  1. The University of Western Ontario, London, Ontario, Canada

    Kaiping Yang

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© 2008 Humana Press, a part of Springer Science+Business Media, LLC

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Jiang, XC. (2008). Generation of Adipose Tissue-Specific Transgenic Mouse Models. In: Yang, K. (eds) Adipose Tissue Protocols. Methods in Molecular Biology™, vol 456. Humana Press. https://doi.org/10.1007/978-1-59745-245-8_4

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  • DOI: https://doi.org/10.1007/978-1-59745-245-8_4

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-916-1

  • Online ISBN: 978-1-59745-245-8

  • eBook Packages: Springer Protocols

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