Summary
Lipolysis involves the sequential breakdown of triglycerides into free fatty acids and glycerol. The extent of lipolysis is therefore a key determinant of the energy status of an individual and also dictates insulin resistance. Here, we describe a protocol for estimating lipolysis in murine adipocytes. Glycerol released during the lipolytic reaction is estimated radiometrically to determine the extent of lipolysis within the cell and the data are normalized to cell number.
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Acknowledgments
This project was supported by the NIDDK/NIH intramural research program.
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© 2008 Humana Press, a part of Springer Science+Business Media, LLC
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Viswanadha, S., Londos, C. (2008). Determination of Lipolysis in Isolated Primary Adipocytes. In: Yang, K. (eds) Adipose Tissue Protocols. Methods in Molecular Biology™, vol 456. Humana Press. https://doi.org/10.1007/978-1-59745-245-8_22
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DOI: https://doi.org/10.1007/978-1-59745-245-8_22
Publisher Name: Humana Press
Print ISBN: 978-1-58829-916-1
Online ISBN: 978-1-59745-245-8
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