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Adipose Tissue Protocols pp 271–283Cite as

Study of Adipose Tissue Gene Expression by In Situ Hybridization

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 456))

Summary

Adipose tissue synthesizes factors involved in the body's homeostasis. Thus, measurements of messenger ribonucleic acid (mRNA) concentrations are important to study the involvement of adipose tissue in various physiological and pathophysiological conditions, in particular in obesity. Because adipose tissue is highly heterogeneous, containing both a stromal and an adipocyte compartment, each one having different cellular composition and functional capacities, in situ hybridization is a powerful tool to analyze the discrete expression of the mRNAs coding for the various factors synthesized within this tissue. Presented here is a detailed protocol for in situ hybridization of mRNAs in adipose tissue using 35S-labeled single-stranded probes with sufficient details for the readers unfamiliar with histologic techniques. Included are details of tissue sectioning and preparation, probe synthesis, hybridization reaction, and macro- and microscopic signal detection.

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Acknowledgments

I thank Dr A Silaghi for her skillful help in photographic art.

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Authors and Affiliations

  1. Inserm U626, Université de la Mediterranée, Marseille, France

    Michel Grino

Authors
  1. Michel Grino
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Editor information

Editors and Affiliations

  1. The University of Western Ontario, London, Ontario, Canada

    Kaiping Yang

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© 2008 Humana Press, a part of Springer Science+Business Media, LLC

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Cite this protocol

Grino, M. (2008). Study of Adipose Tissue Gene Expression by In Situ Hybridization. In: Yang, K. (eds) Adipose Tissue Protocols. Methods in Molecular Biology™, vol 456. Humana Press. https://doi.org/10.1007/978-1-59745-245-8_20

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  • DOI: https://doi.org/10.1007/978-1-59745-245-8_20

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-916-1

  • Online ISBN: 978-1-59745-245-8

  • eBook Packages: Springer Protocols

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