Abstract
Plants are a fabulously rich source of naturally volatile metabolites, which are derived from a range of contrasting biochemical pathways (e.g., mono-, di-, and sesquiterpenoids, benzoates, alcohols, esters). Such volatiles may immediately be released from the plant or they may be stored, e.g., in glycosylated form for release later “on demand.” Certain roles for these molecules have already been determined in that they can function as attractants (e.g., to pollinators, seed dispersers, and others) or as protectants (repellants, pathogen inhibitors, and so on). The flavor and fragrance of plant materials to humans and other animals are also, to a great extent, determined by natural volatiles. Other more sophisticated roles have also been elucidated where plant volatiles have been shown to be involved either as signal molecules to attract the predators of damaging herbivorous insects or potentially even as signal molecules warning other plants of imminent danger. As such, detailed knowledge of these components can be valuable in relation to breeding crop varieties for enhanced product quality or for achieving improved resistance to pathogens and insects. Furthermore, knowledge of the metabolites can result in a corresponding knowledge of the genes responsible for their synthesis and this can lead to dedicated strategies for their in vitro production through, e.g., reverse genetics in heterologous microbial expression systems in fermentors for the production of high-value fine chemicals. Various analytical techniques based on gas chromatography-mass spectrometry have been devised for the analysis of this complex group of metabolites. Two of these key methods are detailed in this chapter.
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References
Seskar, M., Shulaev, V., and Raskin, I. (1998) Endogenous methyl salicylate in pathogen-inoculated tobacco plants. Plant Physiol. 116, 387–392.
Baldwin, E. A., Scott, W. J., Shewmaker, C. K., and Schuch, W. (2000) Flavor trivia and tomato aroma: biochemistry and possible mechanisms for control of important aroma components. HortScience 35, 1013–1022
Augusto, F., Valente, A. L. P., Tada, E. S., and Rivellino, S. R. (2000) Screening of Brazilian fruit aromas using solid-phase microextraction-gas chromatography-mass spectrometry. J. Chromat. A 873, 117–127.
Yang, X. and Peppard, T. (1994) Solid-phase microextraction for flavor analysis. J. Agric. Food Chem. 42, 1925–1930.
Matich, A. J., Rowan D. D., and Banks, N. H. (1996) Solid phase microextraction for quantitative headspace sampling of apple volatiles. Anal. Chem. 68, 4114–4118.
Song, J., Gardner, B. D., Holland, J. F., and Beaudry, R. M. (1997) Rapid analysis of volatile flavor compounds in apple fruit using SPME and GC/time-of-flight mass spectrometry. J. Agric. Food Chem. 45, 1801–1807.
Song, J., Fan, L., and Beaudry, R. M. (1998) Application of solid phase microextraction and gas chromatography/time-of-flight mass spectrometry for rapid analysis of flavor volatiles in tomato and strawberry fruits. J. Agric. Food Chem. 46, 3721–3726.
Augusto, F., Valente, A. L. P., Tada, E. S., and Rivellino, S. R. (2000) Screening of Brazilian fruit aromas using solid-phase microextraction-gas chromatography-mass spectrometry. J. Chromat. A 873, 117–127.
Verdonk, J. C., de Vos, C. H. R., Verhoeven, H. A., Haring, M. A., van Tunen, A.J., and Schuurink, R. C. (2003) Regulation of floral scent production in petunia revealed by targeted metabolomics. Phytochemistry 62, 997–1008.
Verhoeven, H. A., Beuerle, T., and Schwab, W. (1997) Solid-phase micro extraction: artefact formation and its avoidance. Chromatographia 46, 63–66.
Bezman, Y., Mayer, F., Takeoka, G. R., et al. (2003) Differential effects of tomato (Lycopersicon esculentum Mill) matrix on the volatility of important aroma compounds. J. Agric. Food Chem. 51, 722–726.
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© 2007 Humana Press Inc.
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Tikunov, Y.M., Verstappen, F.W.A., Hall, R.D. (2007). Metabolomic Profiling of Natural Volatiles. In: Weckwerth, W. (eds) Metabolomics. Methods in Molecular Biology™, vol 358. Humana Press. https://doi.org/10.1007/978-1-59745-244-1_3
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DOI: https://doi.org/10.1007/978-1-59745-244-1_3
Publisher Name: Humana Press
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Online ISBN: 978-1-59745-244-1
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