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Chromosomal Mutagenesis pp 217–228Cite as

Chromosomal Engineering of Clostridium Perfringens Using Group II Introns

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 435))

Abstract

Clostridium perfringens is a major natural pathogen of human and domestic animals owing to the production of multiple toxins. Defined clostridial mutants are essential for studying the role of toxins in disease pathogenesis. However, it has been very difficult to introduce mutations into C. perfringens. We recently developed a clostridia-modified targetron that can specifically and efficiently inactivate C. perfringens genes. The usefulness of this system has now been demonstrated by specifically inactivating four different C. perfringens toxin genes.

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References

  1. Rood, J. I. (1998). Virulence genes of Clostridium perfringens. Annu. Rev. Microbiol. 52, 333–360.

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  2. Lambowitz, A. M. and Zimmerly, S. (2004). Mobile group II introns. Annu. Rev. Genet. 38, 1–35.

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  4. Fisher, D. J., Miyamoto, K., Harrison, B., Akimoto, S., Sarker, M. R., and McClane, B. A. (2005) Association of β-2 toxin production with Clostridium perfringens type A human gastrointestinal disease isolates carrying a plasmid enterotoxin gene. Mol. Microbiol. 56, 747–762.

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Author information

Authors and Affiliations

  1. Department of Infectious Diseases and Microbiology, Graduate School of Public Health, University of Pittsburgh, Pittsburgh, PA

    Phalguni Gupta & Yue Chen

Authors
  1. Phalguni Gupta
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  2. Yue Chen
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Editor information

Editors and Affiliations

  1. Sigma-Aldrich Biotechnology, Sigma-Aldrich Corporation, St. Louis, Missouri

    Gregory D. Davis

  2. SAFC Biosciences, Sigma-Aldrich Corporation, St. Louis, Missouri

    Kevin J. Kayser

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© 2008 Humana Press Inc., Totowa, NJ

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Cite this protocol

Gupta, P., Chen, Y. (2008). Chromosomal Engineering of Clostridium Perfringens Using Group II Introns. In: Davis, G.D., Kayser, K.J. (eds) Chromosomal Mutagenesis. Methods in Molecular Biology, vol 435. Humana Press. https://doi.org/10.1007/978-1-59745-232-8_16

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  • DOI: https://doi.org/10.1007/978-1-59745-232-8_16

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-899-7

  • Online ISBN: 978-1-59745-232-8

  • eBook Packages: Springer Protocols

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