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Analysis of Lipid Biosynthesis and Location

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Mycobacteria Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 465))

Abstract

A procedure for metabolic labeling of all cellular lipids starting with a culture of mycobacteria is described in this chapter using either a pulse-chase or a simple labeling experimental design. Three fractions are produced for subsequent lipid analysis: (1) the culture filtrate; (2) a readily released surface lipid fraction; and (3) the killed, labeled bacteria. A standardized, TLC-based method for general lipid analysis that can be used to quantify the labeling of all the mycobacterial lipids is given as well as a protocol for analyzing the fatty acyl moieties of the lipids.

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Acknowledgment

P.R.W. was supported through DEFRA funding.

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Authors and Affiliations

  1. Tuberculosis Research Group, Veterinary Laboratories Agency (Weybridge), New Haw, Addlestone, Surrey, KT15 3NB, UK

    Paul Robert Wheeler Ph.D

Authors
  1. Paul Robert Wheeler Ph.D
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Correspondence to Paul Robert Wheeler Ph.D .

Editor information

Editors and Affiliations

  1. Queen Mary's School of Med. & Dentistry, Inst. Cell & Molecular Science, Barts and the London, Newark Street 4, London, E1 2AT, United Kingdom

    Tanya Parish

  2. Queen Mary's School of Med. & Dentistry, Inst. Cell & Molecular Science, Barts and the London, Newark Street 4, London, E1 2AT, United Kingdom

    Amanda Claire Brown

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Wheeler, P.R. (2009). Analysis of Lipid Biosynthesis and Location. In: Parish, T., Brown, A. (eds) Mycobacteria Protocols. Methods in Molecular Biology, vol 465. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59745-207-6_5

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  • DOI: https://doi.org/10.1007/978-1-59745-207-6_5

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-58829-889-8

  • Online ISBN: 978-1-59745-207-6

  • eBook Packages: Springer Protocols

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