Abstract
In this chapter, we describe in detail the steps involved in isolation and characterization of lipoglycans from Mycobacterium tuberculosis and Mycobacterium smegmatis. In addition, procedures involved in structural analysis such as immunoblotting with mAb CS-35 or CS-40, gas chromatography, gas chromatography/mass spectrometry, nuclear magnetic resonance spectroscopy, and endoarabinanase digestion followed by high-pH anion exchange chromatography and two-dimensional gel electrophoresis are presented.
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This work was supported by National Institutes of Health grant AI 37139 and in part from grant AI 52439.
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Shi, L., Torrelles, J.B., Chatterjee, D. (2009). Lipoglycans of Mycobacterium tuberculosis: Isolation, Purification, and Characterization. In: Parish, T., Brown, A. (eds) Mycobacteria Protocols. Methods in Molecular Biology, vol 465. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59745-207-6_3
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DOI: https://doi.org/10.1007/978-1-59745-207-6_3
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